This experiment was carried out to determine the effect of dietary supplementation of Rosemary (Rosmarinus officinalis) ethanol extract, on performance and blood parameters of broilers. One hundred twenty 1-day-old male Ross-308 broiler chicks were used in this experiment. The chickens were divided into 3 groups, including one control and 2 treatment containing 40 birds each. Each group was divided into four replicates and the study lasted 42 days. Group 1 (Control group) was fed with only basal diets. Group 2 (ROE 100mg/kg) was fed basal diet supplemented with 100 mg/kg R. officinalis L. Group 3 (ROE 200mg/kg), was fed basal diet supplemented with 200 mg/kg R. officinalis L. At the end of experiment blood samples collected. Body weight gain (P<0.01) and feed consumption (P<0.001) were decreased when compared with control group, while feed conversion ratio is not affected. Besides, it is detected that rosemary ethanol extract increases the enzymes antioxidant (SOD, GPx, CAT), GSH; reduces lipid peroxidation (MDA) and reduces the ALT, AST, blood glucose level (P>0.05) and LDL-cholesterol (P<0.01). The results of this study showed that lipid peroxidation, oxidative stress parameters significantly decreased after ROE application. Rosemary (R. officinalis L.) ethanol extract at 200 mg/kg administered group was found to create a more positive effect on some biochemical parameters and can be a viable alternative growth promoter in the feeding of broilers.

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