Present study was designed to investigate the level of knowledge and practices for prevention of Bovine Tuberculosis amongst workers at two abattoirs in Karachi. The Data was collected from the herdsman and abattoirs worker at two abattoirs namely City Abattoir Cattle Colony in Landhi and New Karachi slaughterhouse (kamailas) which were working under Karachi Municipal Corporation.120 abattoir workers were interviewed and information was obtained, while frequency counts, percentages and independent sample ttest were used to analyze data. Data indicates the ratio of respondent including herd man and abattoirs workers having no knowledge to those having knowledge was 85:15 Statistical analysis revealed that level of education is potential factor influencing risk of BTB zoonoses amongst abattoir workers Educated respondent were having twice higher knowledge than un educated (OR 0.454, 95% CI 0.236-0.872 P < 0.024).Educated workers demonstrated three time higher practices for preventive measures than non-educated (OR 0.607, 95% CI 0.335-1.102, P< 0.133). Despite having awareness amongst abattoirs worker there was lower level of good practices It was therefore concluded that along with launching awareness program from government organizations it must be ensure practical application of WHO and FAO guidelines for TB free world under one health program ending 2030.

The prevalence of helminthiasis was found to be 15.2 % in buffaloes. Out of 500 samples examined, 9.2 % and 5.4 % were infected with nematodes and trematodes respectively. Mixed infection was observed in 0.6 %.