Background Biological synthesis of natural products from plants has made us an inspiring methodology in the field of science and biotechnology. Methods The methanolic extracts of Catharanthus roseus (L.) G. Don plant leaves (CrPLE) were extracted and characterized by utilizing the phytochemicals estimation, Thin-layer chromatography (TLC), and High-Performance Liquid Chromatography (HPLC) analysis; and further evaluation for an understanding of the biomedical uses of CrPLE was done. Results The evaluation of the seven phytochemicals designates the presence of secondary metabolites in the CrPLE. The CrPLE (test samples) exhibited the Catechin and Caffeic acid contents of 0.0055 and 0.0149 mg/g respectively. The CrPLE revealed the highest antimicrobial activity and showed a mortal effect against the tested microorganisms. Cytotoxicity of the breast cancer cell lines was exposed that CrPLE as a respectable anticancer specialist and metabolically vigorous cells. Conclusion Consequently, the biological synthesized methanolic leaf extracts of the C. roseus plants would be appreciated and have incredible contributions to the field of medicinal applications. Graphical Abstract

Background: Biological synthesis of natural products from the plants has made us an inspiring methodology in the arena of science and biotechnology. Methods: The methanolic leaf extracts of Catharanthus roseus (L.) G. Don plants (CrPLE) were isolated/extracted and characterized utilizing phytochemicals estimation, Thin-layer chromatography (TLC) and High-Performance Liquid Chromatography (HPLC) analysis; and further in understanding the biomedical uses of CrPLE. The evaluation of the seven phytochemicals clearly designates that the presence of secondary metabolites in the CrPLE. Results: The CrPLE (test samples) exhibited the Catechin and Caffeic acid contents of 0.0055 and 0.0149 mg/g respectively. The CrPLE unveiled highest antimicrobial activity and confirmed with mortal effect against the tested microorganisms. A cytotoxicity known against the breast cancer cell lines of exposed that CrPLE as a good anticancer agent and metabolically vigorous cells. Conclusion: Consequently, the biological synthesized methanolic leaf extracts of the C. roseus plants would be appreciated and have the incredible contributions in the field of medicinal applications.