In this research, Trichoderma spp. fungus, a major producer of cellulase, that was isolated from Harran plain had been investigated. Morphological, microscopic and genetic identification of the six fungi isolates were carried out and their cellulase production ability were determined. The ITS region of four of the isolates were displayed over 90% similarity with the DNA sequences of Trichoderma spp. currently deposited into the databases. The molecular size of fungal cellulase was found as to be 32 kDa by SDS-PAGE analysis. Optimum working conditions of cellulase had also been studied. The optimal conditions for cellulase activity determined at 40 °C, pH 5.0, 60 min incubation time and 2% Carboxymethyl Cellulose (CMC). The fungal cellulase activity was compared with the activity of the commercial cellulase enzyme. The fermentation produced enzyme by using Trichoderma spp. isolated from Harran plain displayed remarkable cellulase activity.

Sunflower oil is an oil that is prone to oxidation due to its chemical structure and prevention of such oxidation is widely studied. This study assessed the the effectiveness of  gama glutamyl cyctein (GC) and glutathione (GSH), in preventing oxidation of sunflower oil stored at 50°C for a period of 15 days. Tertiary hydroquinone (TBHQ) was used as a control. Oxidation level indicators such as peroxide (PV), free fatty acidity (FFA), p-anisidine (p-AV) but also total oxidation (Totox), colour (L*, a*, b*) and free fatty acid profile were determined. At the end of storage, oxidation in sunflower oil was substantially reduced by 40 mg/L of GC. Analysis with 2,2-diphenyl-1-picrylhydrazyl (DPPH) resulted in the following order of antioxidant activities: TBHQ>BHA>GC>GSH>BHT. The samples resistance to the generation of primary and secondary oxidation products was T>GC+T>GC>GSH+T>GSH>C for up to 15 days under storage conditions. The free fatty acid profile analysed by GC/MS further demonstrated that these thiols outperformed the control group in terms of performance. Findings demonstrated that GC, precursor of GSH, has stronger antioxidant activity than GSH. As a result, it is recommended to be explored as a potential source of antioxidants in applications for the food industry to prevent lipid oxidation.