Covid-19 or Corona virus disease spreading since December 2019, starting from Wuhan China has now become a pandemic throughout the globe. For early detection of the infected persons prior to the onset of the disease has become necessary to stop the spreading of this communicable disease. The process of testing of respiratory Covid-19 and the associated SARS- COV-2 virus is proved to be possible only by the detection of antibody produced due to response to the infection. This process of antibody detection can be used for both diagnosis and population surveillance. The testing of generated antibodies due to SARS-COV-2 are antibody isotopes that are to be detected are IgG and IgM. Due to having novel antigens, the first responding antigen antibody interactions was IgM. Antibodies that show a higher affinity for more specifically binding capable antigens leading to the proper immune response is IgG. Due to the infectious reactions, IgG antibodies are produced. The positiveness of a sample is considered if both IgG and IgM are present. Whole blood, serum or plasma specimens are used for membrane based immunoassay in a qualitative IgG and IgM test kit. The principle for the testing is the same that of a HCG pregnancy test where human glycoprotein is assayed in a rapid chromatographic way. The mentioned process is very useful for mass detection of the Covid-19 infected population in a minimum time and is also safe for the laboratory technicians as it can easily be done without getting infected if proper physical barrier is taken personally.
Antihyperglycemic activity of leave extracts of Ficus carica was evaluated on STZ induced diabetic rats. Diabetes was induced in albino Wistar rats of either sex by intraperitoneal (60mg/kg b.w.) of STZ, freshly dissolved in citrate buffer (0.01 M, pH 4.5). Ficus carica leave extract in different solution (viz. petroleum ether, ethyloacetate, methanol and aqueous) were administered to diabetic rats for 9 days. The effect of extracts on blood glucose and body weight was studies on day 1st and 9th. The study showed that the ethyl acetate, methanolic and aqueous extract of Ficus sarmentosa leaves reduced blood glucose level and body weight significantly. This may justify the use of ficus species as ethanomedical medicine for treatment of diabetes mellitus.
Paclitaxel (PTX) obtained from the bark of Taxus brevifolia (Pacific yew tree) is a well-known potent drug used for treatment of breast, lung and ovarian cancer. PTX is stated to be a novel antimicrotubule agent. PTX acts by assembling the microtubule from tubulin dimmers and stabilizing of microtubules by prevention of polymerization. Thus it affects the cell division of the cancer cells by interrupting the spindle formation. PTX on application in cancer treatment it shows to have low aqueous solubility use of vehicles like Cremophore EL and ethanol, which on application shows hypersensitivity reaction. So to reduce the toxicity due to these vehicles three main dosage forms are seen to be developed for application of PTX in chemotherapy by researchers throughout the world. Namely; Nano-Particle Approaches, Liposomal approach, Solid Dispersion approach. Nanoparticles are utilized for purposes like decreasing toxicity and minimizing adverse effects of drug molecules and enhancing drug release. Liposomes are capable of containing more amount of PTX and are capable of long term stability, toxicity reduction when compared to other dosage form. Solid dispersions are more effective compared to other methods of particle size reduction to improve the drug solubility. So it can be stated that developing dosage forms like these for reduction of toxicity and efficacious application of PTX in chemotherapy is important.
As we know novel coronavirus is an emergent nuisance in this stipulated period. Corona virus is a group of enveloped viruses, with non-segmented, single stranded & positive sense RNA genomes. Human Corona virus is mainly subdivided into four categories such as 229E, NL63, OC43, HKU1. Epidemiologically it has a greater prevalence in the modern era. The features encountered in the clinical course of the disease are multifarious spanning from cough, sneezing, fever, breathlessness. It may take 2-14 days for a person to notice symptoms after infection. Azithromycin and 8 Hydroxychloroquine both plays an instrumental role for management of COVID-19. Azithromycin is a macrolide antibiotic and it binds with a 50s ribosome then inhibits bacterial protein synthesis. On the other hand 8-Hydroxychloroquine was approved by United State in the year of 1955 .Basically it is used as a antimalarial drugs . Briefly, in inflammatory conditions it binds with toll like receptor & blocks them. 8- hydroxychloroquine increases lysosomal pH in antigen presenting cells . In inflammatory conditions it blocks toll like receptors on plasmacytoid dendritic cells. In our review we focused on the role of Azithromycin, and 8-hydroxychloroquine in Covid-19 .
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