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Summary Nowadays, cosmetic as well as pharmaceutical forms must undergo many controls, i.e. in-process controls on the finished product and stability testing. The authors suggest using first derivative spectroscopy for active ingredients assay presenting an ultra-violet absorption. It offers the advantage of a direct assay through simple dilution and without active ingredient extraction. Tests were made on classic mixtures of the sunscreens benzimidazol and cinnamate derivative (pair 1), and a mixture of two benzophenones (pair 2). The studied forms were solutions in propylene glycol and commercialized W/O and O/W creams. After analytical method validation for each sunscreen by precision, reproducibility and repeatability studies, the percentages of error of the various assays have been reported in various preparations (solutions or creams). A comparative study between HPLC assay (traditionally used) and the proposed method was carried out. The results show that a highly significant linear correlation exists between the two methods for the four sunscreens (R > 0.992). The speed and simplicity of the first derivative spectrometric method should find applications in routine control or in development of cosmetic or pharmaceutical preparations.

In this present project, a novel, Reverse Phase -High Performance Liquid Chromatographic analytical method was developed for determination of Fulvestrant Injection, which is fast & economical too. Retention time of Fulvestrant was at 21 minutes, which quite fast by using the Zorbax XDB C18; 150 × 4.6 mm, 3.5, column as stationary phase with mobile phase consisted of a mixture of Mobile phase -A: Water, Acetonitrile and Methanol (410:320:270), Mobile phase-B: Acetonitrile, Methanol and Water (490:410:100) in a gradient elution and at a flow rate of 2 ml/min. Detection was carried out at 225 nm in HPLC. Newly developed method shows linearity in the range of 80-120 μg/ml & correlation co-efficient for this method was found to be 0.999. The accuracy studies showed % recovery of Fulvestrant injection, was in the range 99.7-102 % in the newly developed method. Validation parameters were within the permitted limits so this method was found to be precise, accurate and specific. Present method is better in terms of economical aspect, easy to perform & is very much specific towards the targeted drug, which is evidenced from the validation parameter. So this unique method can be efficiently employed for determination of Fulvestrant in commercial products, economically.

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Ulcerative colitis (UC) is a chronic ulceroinflammatory condition primarily confined with in colonic mucosa with variable distortion of the colonic architecture. It is associated with microbial overgrowth such as E.coli , S. typhi .However Chloroquine acts on these microbes. In our present study the anticolitic effects of Chloroquine in treatment with the DSS induced model for UC in mice were examined. Male mice of age 7 weeks are chosen .They are assigned into different groups .Negative control groups treated with normal saline solution ,positive control group treated with DSS5%, standard group treated with Balsalazide 4mg/kg & DSS 5% , Test(1) group treated with Chloroquine 6mg/kg, Test(2) group treated with Chloroquine 8mg/kg, Test(3) group treated with Chloroquine 10 mg/kg then we performed different parameters such as DAI, Oxidative damage assessment, MPO Assessment and Histopathological evaluation. The results have showed that Chloroquine has significant activity against DSS(5%) induced colitis when compared to the experimental control, with near normalization of colon architecture. Tissue oxidative stress was reduced with significant improvement in tissue levels of MDA, GSH, SOD and CAT .Furthermore,significant improvement in levels of myeloperoxidase (MPO) was observed. It is concluded that Chloroquine(10mg/kg) has got potent activity against DSS(5%) induced ulcerative colitis(UC) due to its anti inflammatory antioxidant properties.