This study derives from the macroscopic analysis of a Late Neolithic population from Hungary. Remains were recovered from a tell settlement at Hódmezővásárhely-Gorzsa from graves within the settlement as well as pits, ditches, houses and as stray finds. One of the most important discoveries from these remains was evidence of tuberculosis. Pathological analysis of the seventy-one individuals revealed numerous cases of infections and non-specific stress indicators on juveniles and adults, metabolic diseases on juveniles, and evidence of trauma and mechanical changes on adults. Several cases showed potential signs of tuberculosis and further analyses were undertaken, including biomolecular studies. The five individuals were all very young adults and included a striking case of Hypertrophic Pulmonary Osteopathy (HPO) with rib changes, one case with resorptive lesions on the vertebrae, two cases with hypervascularisation on the vertebrae and periosteal remodelling on the ribs, and one case with abnormal blood vessel impressions and a possible lesion on the endocranial surface of the skull. The initial macroscopic diagnosis of these five cases was confirmed by lipid biomarker analyses, and three of them were corroborated by DNA analysis. At present, these 7000-year-old individuals are among the oldest palaeopathological and palaeomicrobiological cases of tuberculosis worldwide.

Seventy-one individuals from the late Neolithic population of the 7000-year-old site of Hódmezővásárhely-Gorzsa were examined for their skeletal palaeopathology. This revealed numerous cases of infections and non-specific stress indicators in juveniles and adults, metabolic diseases in juveniles, and evidence of trauma and mechanical changes in adults. Several cases showed potential signs of tuberculosis, particularly the remains of the individual HGO-53. This is an important finding that has significant implications for our understanding of this community. The aim of the present study was to seek biomolecular evidence to confirm this diagnosis. HGO-53 was a young male with a striking case of hypertrophic pulmonary osteopathy (HPO), revealing rib changes and cavitations in the vertebral bodies. The initial macroscopic diagnosis of HPO secondary to tuberculosis was confirmed by analysis of Mycobacterium tuberculosis complex specific cell wall lipid biomarkers and corroborated by ancient DNA (aDNA) analysis. This case is the earliest known classical case of HPO on an adult human skeleton and is one of the oldest palaeopathological and palaeomicrobiological tuberculosis cases to date.

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Ajdovska Jama (The Pagan's Cave) in southeast Slovenia lies within the catchment of the River Sava, a major tributary of the Danube. The site is well known for its Neolithic burials and has been excavated to a high standard on various occasions since 1884. The human remains at the site occurred as distinct clusters of mainly disarticulated bones belonging to at least 31 individuals. Hitherto, dating of the burials has been based on the associated archaeological finds, including a few low-precision radiometric radiocarbon measurements on charred plant material. In the present study, bones from 15 individuals were subsampled for accelerator mass spectrometry (AMS) and stable isotope analyses. These comprised adults and children from 3 of the clusters. The results of the study indicate that the burials all belong to a relatively short time interval, while the stable isotope data indicate a mixed diet based on C3 plant and animal food sources. These interpretations differ somewhat from those of previous researchers. The AMS 14C and stable isotope analyses form part of a wider investigation of dietary and demographic change from the Mesolithic to the Iron Age in the Danube Basin.