Hypersensitivity, local irritative and cytotoxic effects are known for the chemical components of Syzygium aromaticum and Cinnamomum zeylanicum contained in dental materials. However, there is no intimate data in dentistry using the whole extracts of these plants and introducing new ones. Salvia triloba is a well-known anti-inflammatory plant that correspondingly could be used in several dental traumas.Objectives:We aimed to show and compare the effect of S. aromaticum, C. zeylanicum, and S. triloba extracts on dental pulp stem cells (DPSCs) proliferation, differentiation, and immune responses.Material and Methods:Using xCELLigence, a real time monitoring system, we obtained a growth curve of DPSCs with different concentrations of the Extracts. A dose of 10 μg/mL was the most efficient concentration for vitality. Osteogenic differentiation and anti-inflammatory activities were determined by using an ELISA Kit to detect early and late markers of differentiation.Results:The level of osteonectin (ON, early osteogenic marker) decreased, which indicated that the osteogenic differentiation may be accelerated with addition of extracts. However, the level of osteocalcin (OCN, late osteogenic marker and sign of calcium granulation) differed among the extracts, in which S. aromaticum presented the highest value, followed by S. triloba and C. zeylanicum. Surprisingly, the determined calcium granules were reduced in S. aromaticum and S. triloba. In response to tumor necrosis factor alpha (TNF-α), S. triloba-treated DPSCs showed the most reduced level of IL-6 cytokine level. We suggest C. zeylanicum as a promising osteogenic inducer and S. triloba as a potent anti-inflammatory agent, which could be used safely in biocomposite or scaffold fabrications for dentistry.Conclusions:Because calcium granule formation and cell viability play a critical role in hard tissue formation, S. aromaticum in dentistry should be strictly controlled, and the mechanism leading to reduced calcium granule formation should be identified.

A natural agent that maintains mesenchymal stem cell (MSCs)

It is a big issue that reduced bone density and large fractıres in dentistry and orthopedics. Side effects caused by synthetic drugs lead to medical and ethical problems. Thus, plants and medicinal plant research take attention. Aim of this preliminary in vitro study is to investigate the effect of Ocimum basilicum extract on dental pulp (DP) and bone marrow (BM) derived mesenchymal stem cell (MSC) proliferation, osteogenic differentiation and immunological response to TNF-α. Human dental pulp tissue was obtained from patients (15-20 years of age) who were undergoing extraction of third molars for orthodontic reasons at the Department of Oral and Maxillofacial Surgery, University of Gazi University * . xCELLigence system was used to determine prolfieration of DP-and BM-MSCs. Adipogenic and osteogenic differentiation was shown and calcium concentration, osteocalcin and osteonectin levels were examined. Inflammatory environment was mimiced through TNF-α stimulation and IL-6 and IL-10 levels were defined by ELISA. Doubling time mwith O. basilicum was found in DP-MSCs (38 h) and BM-MSCs (76 h). IC50 value was shown as 148 µg/mL in DP-MSCs and 178 µg/mL in BM-MSCs. Calcium concentration of BM-MSCs was found decreased in O. basilicum treated groups. Level of ostoenectin was reduced in O. basilicum treated cells suggesting that the Extract accelerated the osteogenic differentiation. We suggest that O. basilicum could be a smart ostoeinductive agent where BM-MSCs should be investigated further. Rich flora of Turkey is an opportunity for us and encourangement can easily give inside to medicinal plant investigations. * B.30.2.GÜN.0.20-122 Ethics Committee Report

The aim of this study is to demonstrate and compare the differentiation, proliferation, migration and inflammatory behavior of dental pulp- and bone marrow-derived mesenchymal stem cells (DP-MSCs and BM-MSCs) in response to a Hypericum perforatum ethanol extract. Using xCELLigence, a real-time monitoring system, a dose of 10 µg/mL was found to be the most efficient concentration for vitality. The IC50 values and doubling time were calculated. The results showed that H. perforatum L. was able to accelerate osteogenic differentiation in DP-MSCs, but calcium granulation was impaired in BM-MSCs. H. perforatum L.-induced migration increased when compared to the TNF-α-induced migration in a Transwell migration assay, and the IL-6 cytokine levels between cells also differed. It can be suggested that tissue memory is an important factor in MSCs, and that they differ in their response to external factors. In conclusion, H. perforatum L. can be considered an excellent osteoinductive agent for DP-MSCs but should not be used for BM-MSCs. Tissue-specific osteoinductive agents should be discussed in future studies.

Amaç: Künt göz travmalarında sonuç en iyi düzeltilmiş görme keskinliği (EDGK) üzerine etkisi olan faktörleri belirlemek. Ge reç ve Yön tem: S.B. Ankara Eğitim ve Araştırma Hastanesi 2. Göz Kliniği Acil Bölümüne Haziran 2007-Ağustos 2008 tarihleri arasında künt göz travması ile başvuran 142 hastanın 142 gözü son ölçülen EDGK ile buna etki eden faktörler ve ortaya çıkan komplikasyonlar açısından prospektif olarak çalışmaya dahil edildi. Sonuçlar: Hastaların yaşları 3 ile 76 arasında değişmekte olup ortalama yaş 31,9±17,6 yıl idi. Olguların 107'si (%75,4) erkek, 35'i (%24,6) kadındı. Son ölçülen EDGK 120 olguda log MAR ≤1,00 (%84,5), 22 olguda log MAR >1,00 'de (%15,5) olduğu görüldü. Tek yönlü varyans analizine göre, son ölçülen EDGK'nin kötü seyretmesi üzerinde, ilk görme keskinliği (p=0,039), kesi büyüklüğü (p=0,029), rölatif afferent pupil defekti (RAPD) (p<0,001), etkilenen zon (p<0,001), arka segment bulgusu (p=0,001.) ve okuler travma skorunun etkisi (OTS) (p<0,001) istatistiksel olarak anlamlı bulundu. Tar t›fl ma: Künt göz yaralanması, her yaşta hasta grubunu etkileyebilen kalıcı görme azlığının önlenebilir bir sebebidir. Her hastaya sistematik ve doğru bir tedavi yaklaşımının uygulanabilmesi için son ölçülen EDGK üzerinde etkisi olan faktörlerin bilinmesi önemlidir. İlk görme keskinliği, kesi büyüklüğü, RAPD varlığı, etkilenen zon, arka segment bulgusu ve düşük OTS değeri, son ölçülen EDGK'nin kötü seyretmesi üzerinde etkisi istatistiksel olarak anlamlı bulunmuştur. (Turk J Ophthalmol 2013; 43: 32-8) Anah tar Ke li me ler: Künt göz travması, oküler travma skoru Sum mary Pur po se: To determine the factors affecting the outcome of best-corrected visual acuity (BCVA) in blunt ocular trauma cases. Ma te ri al and Met hod: 142 eyes of 142 patients, who were admitted to the 2nd Eye Clinic Emergency Service of M.H. Ankara Training and Research Hospital between June 2007 and August 2008, with blunt ocular trauma were prospectively included in the study in terms of the last-measured BCVA, the factors affecting it, and the ensuing complications.Re sults: Patient age ranged from 3 to 76 years (mean age: 31.9 ± 17.6 years). One hundred and seven cases (75.4%) were male and 35 (24.6%) were female. The last-measured BCVA in 120 cases (84.5%) was found to be log MAR ≤ 1.00 and in 22 cases (15.5%) was log MAR > 1.00. According to the one-way analysis of variance, the effect of initial visual acuity (p = 0.039), incision size (p = 0.029), relative afferent pupillary defect (RAPD) (p<0.001), zone (p <0.001), posterior segment findings (p= 0.001.), and ocular trauma score (OTS) effect (p <0.001) on worsening of the last-measured BCVA were statistically significant. Dis cus si on: Blunt eye injury is a preventable cause of permanent visual loss, which can affect people of all ages. It is important to know the factors affecting the last-measured BCVA for the implementation of a systematic and accurate treatment approach to each patient. The effect of initial visual acuity, the size of the incision, the presence of RAPD, zone,...