Extract of Stevia leaf of rebaudiana Bert has a content that is easily oxidized by the presence of light and heating so it is not stable during storage. A microencapsulation process is carried out which aims to protect the contents in the extract. This study aims to determine the effect of storage temperature on the physical stability of the microencapsulation of Stevia leaf of rebaudiana Bert with a combination of encapsulants inulin chitosan. A study was conducted to determine the effect of temperature on the microencapsulation stability of Stevia leaf of rebaudiana Bert over a period of 1 month. Tests carried out on the microencapsulation of Stevia leaf of rebaudiana Bert include the examination of the physical parameters of the microcapsules, namely the microencapsulation yield, moisture content, solubility, flow rate, non-encapsulated and encapsulated extracts content and determination of particle distribution and morphological form. The test results show that the storage temperature does not affect the microencapsulation stability of Stevia leaf of rebaudiana Bert, because the SEM test results did not change i.e. the particle size distribution of 1 -1000 µm. The average yield obtained from making microencapsulation of Stevia leaf extract of rebaudiana Bert was 98.20%. Storage temperatures of 5˚C, 25˚C and 40˚C affect the physical stability of the microencapsulation of Stevia leaf extract of rebaudiana Bert due to changes in the profile of physical characteristics during storage.
Diabetes mellitus is a collection of symptoms that arise in someone who has increased blood glucose levels. The Stevia plant (Stevia rebaudiana Bert) contains a compound of diterpene glycosides as steviosida and rebaudiosida A. Purified extract of steviosida and rebaudiosida A is widely used as a sweetener for low calorie food and beverage products or as a sugar substitute for diabetics and has the effect of lowering blood sugar levels. This study aims to determine the antidiabetic effect of microencapsulated preparations of Stevia leaf extract (Stevia rebaudiana) with a combination of inulin chitosan encapsulation. Antidiabetic mellitus test was carried out in vivo using test animals of male white rats of Wistar strain. The inducing compound that can cause the condition of diabetes mellitus test animals is Aloxan with a dose of 150 mg / kg Body weight of rats. given intraperitoneally for one day, then the mice were allowed to stand for 3 days to reach a state of diabetes mellitus. Blood glucose levels of test animals were measured on days 1 (initial), 5 (induction) and 12 (treatment) to determine the initial blood glucose levels, after induction of alloxan and after administration of test compounds both CMC Na 0.5% , glibenclamide, and preparations microencapsulation of Stevia leaf extract at a dose of 100; 300; and 700 mg / kg body weight. The results were obtained after 7 days of treatment, it was seen that blood glucose levels in the CMC group remained high, while the Glibenclamid administration group, and the three dosages of microencapsulation preparations of Stevia leaf extract could reduce blood glucose levels. This can be seen from the statistical test that there is a significant difference (p
Validation of methods is necessary to ensure that the analytical methods used are appropriate and reliable. In this research, the determination of validation method and determination of inulin content from gembili bulb (Dioscorea esculenta L.). The method used for determining inulin levels is UV-Vis spectrophotometry. Based on the results obtained, the maximum wavelength determination of inulin levels is 520 nm. The method of analysis in this study meets the specified validation requirements. These parameters include the equation on the inulin raw curve y = 0.0045x +0.109 with the coefficient of determination 0.9992. The accuracy of the methods tested using the recovery test was 96.14-106.76% at concentrations of 25, 100, and 225 ppm. The intraday precision test is in the range of 2.67-6.82% at the same concentration as the accuracy test. Precision interday equal to 7.41% at concentration 225 ppm for 3 day analysis period. The values of LOD and LOQ are 3.58 ppm and 11.95 ppm, respectively. The concentration of inulin extract from gembili bulb is 840.44-12188.59 ppm.
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