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The conjugated polymers studied as materials of biosensors are sensitive to external stimuli. The conjugated polymer is superior in thermal properties to low molecular weight materials and is widely used as a material for detecting heavy metals, and noxious gas because it is easily applied to thin films and the like (Son et al. 2015). Polydiacetylene (PDA) is a conjugated polymer that has an intersection between a double bond and a triple bond. In addition, PDA can be synthesized easily by polymerizing diacetylene monomers through UV light irradiation, free radical polymerization, or plasma treatment (Okada et al. 1998; Lee et al. 2014; Lu et al. 2014). Furthermore, the advantage of diacetylene monomer in the aqueous state is that it has the ability to self-assemble into a three-dimensional structure. The wavelength of the self-assembled, three-dimensional structure is similar to that of a polymer intersecting with a triple

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