Grapes are one of the most widely grown fruit around the world but is not for tropical climates area. However, currently in Malaysia the grapes planting become more popular due to the successful production from the local farmers. Due to this situation, the researchers start to focus on the product development from the grapes. This research is to screen the phytochemical compound of grapes leaves extract from Jupiter variety and their antioxidant and insecticidal activity. The DPPH test method was used to determine the antioxidant level. Folin-Ciocalteau assay and aluminium chloride colorimetric assay were used to assess total phenolic and flavonoid content, respectively. The phytochemical was also screened to determine the compound presented in extract. Then, the extract was tested on the Sitophilus oryzae to examine the insecticidal activity in stored rice. The result showed that extract of has a good potential in order to control Sitophilus oryzae and has a high amount of antioxidant. The various compounds presented in the extract that led to the high insecticidal activity. Thus, this result is very significant to help the rice producer in order to control the beetles in rice storage without reducing the quality of rice.
The broad-leaved weeds Melastoma malabathricum, Clidemia hirta, Chromolaena odorata, and Ageratum conyzoides are characterized as potential weeds due to their high nutritional value and abundance of chemical components for different bioactivities. GC-MS analysis was used in the current study to identify potential bioactive components, mainly for insecticidal activity. Extraction from the leaves of these weeds was done using different solvents like ethanol, acetone, and ethyl acetate. The findings of the GC-MS analysis revealed that the ethyl acetate extract, which has the largest peak area (93.23%), contains the major of the compounds present in the M.malabathricum leaf extract. GC-MS analysis of C.hirta revealed that the major compound is in the ethyl acetate extract with a 50.05% peak area. C.odorata showed the major compound in the ethanol extract with a 70.13% peak area. A.conyzoides shows that the major compound is in acetone extract with a 29.42% peak area. These are the compound name respectively. Methyl 7-octadecenoate for M.malabatrichum, 2-Hexadecen-1-ol, 3,7,11,15-tetramethyl for C.hirta, Palmitic acid, ethyl ester for C.odorata and α-Santalene for A.conyzoides. Some types of compounds found are known to have the potential as antibacterial, antiviral, antioxidant, insecticidal, and others.
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