Grapes are one of the most widely grown fruit around the world but is not for tropical climates area. However, currently in Malaysia the grapes planting become more popular due to the successful production from the local farmers. Due to this situation, the researchers start to focus on the product development from the grapes. This research is to screen the phytochemical compound of grapes leaves extract from Jupiter variety and their antioxidant and insecticidal activity. The DPPH test method was used to determine the antioxidant level. Folin-Ciocalteau assay and aluminium chloride colorimetric assay were used to assess total phenolic and flavonoid content, respectively. The phytochemical was also screened to determine the compound presented in extract. Then, the extract was tested on the Sitophilus oryzae to examine the insecticidal activity in stored rice. The result showed that extract of has a good potential in order to control Sitophilus oryzae and has a high amount of antioxidant. The various compounds presented in the extract that led to the high insecticidal activity. Thus, this result is very significant to help the rice producer in order to control the beetles in rice storage without reducing the quality of rice.

The use of synthetic fungicide are affecting the health, disturbing the ecosystem of the environment and has develop resistance of many pathogenic fungi. This study aimed to investigate the antifungal activity of ethanolic Piper betel (P. betel) crude leaf extracts with HOCl formulated by natural oxygenated water against selected crop pathogenic fungi which are Fusarium spp. and Phakopsora euvitis fungi. The screening test was done using well diffusion technique where the parameter observed was the Percentage Inhibition Radial Growth (PIRG). The antifungal effects of P.betel crude leaf extract with formulated HOCl minimum inhibitory concentration (MIC) were further evaluated by micro broth dilution method and minimum fungicidal concentration (MFC) were tested by agar well method. Screening results showed the P.betel extracts with HOCl have potential to inhibit the fungi radial growth when compared to positive control Kencozeb M45. MIC and MFC results portrayed the extracts with formulated HOCl displayed fungicidal activity against both fungi in the range of 2500 µg/ml until 5000 µg/ml for Fusarium spp and Phapkopsora euvitis, respectively. To the best of our knowledge this is the only natural oxygenated water source in Malaysia and first reported with combination of Piper betel extract.