Chlorella is included in a group of an autotrophic protococci alga and is occurred in ponds, wells, moist soil and on the surface of trees. It is circular in shape, 1.5-10.5μm in size and is a unicellular alga (1). Among them, some strains of Chlorella genus contain 45-50% protein, 20- 25% carbohydrate, 7-10% lipids, and biologically active substances such as several types of vitamins and essential amino acids, hence, other countries are widely utilizing them in food, forage, pharmaceutical and cosmetic purposes (1.7). Other countries are searching for chances to isolate a microalgae strain from their natural resources which can synthesize biologically active substances, to determine their properties, to utilize them practically and to produce their biopreparations by biotechnological methods (1.2.7). Therefore, we studied the physiological and biochemical properties of the cultures that were isolated from the soil of a greenhouse in order to detect a biologically active microalgae strain.DOI: http://dx.doi.org/10.5564/pmas.v0i4.51 Proceedings of the Mongolian Academy of Sciences 2009 No.4 pp.86-89