Chlorella is included in a group of an autotrophic protococci alga and is occurred in ponds, wells, moist soil and on the surface of trees. It is circular in shape, 1.5-10.5μm in size and is a unicellular alga (1). Among them, some strains of Chlorella genus contain 45-50% protein, 20- 25% carbohydrate, 7-10% lipids, and biologically active substances such as several types of vitamins and essential amino acids, hence, other countries are widely utilizing them in food, forage, pharmaceutical and cosmetic purposes (1.7). Other countries are searching for chances to isolate a microalgae strain from their natural resources which can synthesize biologically active substances, to determine their properties, to utilize them practically and to produce their biopreparations by biotechnological methods (1.2.7). Therefore, we studied the physiological and biochemical properties of the cultures that were isolated from the soil of a greenhouse in order to detect a biologically active microalgae strain.DOI: http://dx.doi.org/10.5564/pmas.v0i4.51 Proceedings of the Mongolian Academy of Sciences 2009 No.4 pp.86-89
The symbiotic system of Legumes and root-nodule bacteria is a significant process for enriching barren and dry soils and supporting plant growth. The aim of this study was to isolate root-nodule bacteria from Medicago falcate and to select high activity strains with resistance to environmental stress. As a result, only MSZ-25 strain grew in the medium containing 0.8M NaCl, with pH4.8-9.8 and at temperatures of 4-45°С. All rhizobial isolates were tolerant to the concentrations of 0.01-0.1% Pb(NO3)2, 0.01% ZnSO4, CdCI2, CuCI2 and NiCI2. The strains were also resisted to penicillin 10μ/ml, erythromycin 10μ/ml, and chlorampenicol 10μ/ml but the growths of all isolates were inhibited by kanamycin 30μ/ml, novobiocin 30μ/ml and tetracycline 10μ/ml.
Rhodotorula glutinis is an aerobic yeast with particular metabolic characteristic that can produce large amount of carotenoids during the stationary growth phase. Carotenoid is an important natural pigment with antioxidant properties, which is used in food, pharmaceutical and cosmetics industry as additives. The aim of this work is to study the production of carotenoid with antioxidant activity by R.glutinis R12 in different media conditions. The selected strain was cultivated in three different culture media such as YM broth, Basal and MS3 medium at 28°C for 72–120 hours. After fermentation, cells were harvested by centrifugation and freeze-dried. Carotenoid from the biomass was extracted as a mixture of DMSO, acetone and petroleum ether with a ratio of 1:2:2 and cells were ruptured using ultrasonic wave. The carotenoid content in the supernatant was measured by spectrophotometric method. The highest content of carotenoids extracted from R.glutinis R12 was 283.71µg/g dry biomass. The maximum antioxidant activity of carotenoid by DPPH assays were achieved 52.09 ± 0.4% (IC50= 536.02) and at a concentration of 600 µg ml-1. This study revealed that the R.glutinis R12 strain has the ability to produce carotenoid and has shown antioxidant activity in Basal and MS3 medium. For further study, it is necessary to investigate the improvement of carotenoid yield from R.glutinis R12 strains.
ОРШИЛRhizobium -буурцагт ургамлын үндсэнд симбиоз үүсгэн оршдог, генетикийн хувьд олон бүлэгт хамаарах Грам сөрөг бичил биетэн юм. Эдгээр нь агаарын азотыг шингээн ургамлыг тэжээлийн бодисоор хангаж, хөрсийг азотоор баяжуулахаас гадна буурцагт ургамлыг хортон шавьж, өвчин үүсгэгчээс хамгаалах, орчны таагүй нөхцөлд дасан зохицох чадварт эерэг нөлөө үзүүлдэг. Буурцагтны булцууны бактерийн симбиоз үүсгэлт гадаад орчны хүчин зүйлс болох температур, чийг, хөрсний рН, давсны концентрациас ихээхэн хамаардаг [8,10,11].Lupinus albus L буюу Цагаан шошлойны үр болон ногоон масссыг гэрийн тэжээвэр тахиа, гахайнд уурагт тэжээл болгон дэлхийн олон оронд өргөн хэрэглэж байна. DOI: http://dx.doi.org/10.5564/pmas.v56i3.693 Цагаан шошлойн үрийн найрлагад 37-42% уураг, 10-12% тос, 7% эслэг агуулдаг, үрийн шинж чанараар шар буурцагтай ойролцоо боловч хөрс, цаг уурын нөхцөлд илүү мэдрэг. 1960-аад оноос эхлэн Гатаулина болон бусад эрдэмтэд Lupinus albus L -ийн ургалтын хугацааг богиносгох, өвчин үүсгэгчид тэсвэртэй болгох (фузариоз болон антракноз), ургацыг нэмэгдүүлэх, үрийн чанарыг сайжруулах чиглэлээр селекцийн ажил явуулсны дүнд олон сорт гарган авсан [4,5].ОХУ-д 2004 онд бүртгэгдсэн Дега сортыг тэжээлийн эх үүсвэр болгон хамгийн өргөн хэрэглэж байна. Дега сортын вегетацийн хугацаа 115-130 хоног, өвчин үүсгэгч мөөгөнцөрт тэсвэртэй, үрийн найрлагадаа 8-10% тос, 37-38% уураг, ногоон массдаа
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