Scaffold proteins link signaling molecules into linear pathways by physically assembling them into complexes. Scaffolds may also have a higher-order role as signal-processing hubs, serving as the target of feedback loops that optimize signaling amplitude and timing. We demonstrate that the Ste5 scaffold protein can be used as a platform to systematically reshape output of the yeast mating MAP kinase pathway. We constructed synthetic positive- and negative-feedback loops by dynamically regulating recruitment of pathway modulators to an artificial binding site on Ste5. These engineered circuits yielded diverse behaviors: ultrasensitive dose response, accelerated or delayed response times, and tunable adaptation. Protein scaffolds provide a flexible platform for reprogramming cellular responses and could be exploited to engineer cells with novel therapeutic and biotechnological functions.

The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae.

Many properties of an optically interconnected system can be improved through the use of a modelocked laser. The short pulse duration, high peak power, wide spectral bandwidth, and low timing jitter of such a laser lead to these benefits. Timing advantages include simplified synchronization across large chip areas, receiver latency reduction, and data resynchronization. Lower power dissipation may be achieved through improved receiver sensitivity. Additional applications of short optical pulses include time-division multiplexing, single-source wavelength-division multiplexing, and precise time-domain testing of circuits. Several of these concepts were investigated using a high-speed chip-to-chip optical interconnect demonstration link. The link employs a modelocked laser and surface-normal optoelectronic modulators that were flip-chip bonded to silicon CMOS circuits. This paper outlines experiments that were performed on or simulated for the link, and discusses the important benefits of ultrashort optical pulses for optical interconnection.

Abstract-We present a new technique of injecting clocks optically onto CMOS chips without the use of a receiver amplifier. We discuss the benefits of such a direct approach and present proof-ofprinciple experiments of the technique. We analytically compare a receiver-less optical clock distribution and an electrical clock distribution in a fan-out-of-four clock tree to evaluate the timing and power benefits of the optical approach for present microprocessors. We also compare receiver-less direct injection of optical clocks to trans-impedance receiver based injection within the same distribution framework.

An electroabsorption modulator using a side-entry architecture achieved a contrast ratio exceeding 3 dB over a 3.5 nm range in the C-band, using a voltage swing of 1 V and operating at 1008C. Modulation was due to the quantum-confined Stark effect from ten Ge/SiGe quantum wells epitaxially grown on silicon-on-insulator (SOI) wafers. The device exploits an asymmetric Fabry-Perot resonator formed between the totally internally reflecting air-SiGe interface and a frustrated total internal reflection from the buried oxide layer of the SOI substrate.