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A range of Cu-LDHs has been synthesized by co-precipitation using metal nitrate precursors and sodium carbonate under varying molar ratios Cu/Al (Cu 0.05 -Al 0.15 , Cu 0.10 -Al 0.10 , Cu 0.14 -Al 0.06 , and Cu 0.15 -Al 0.05 ). The uncalcined and calcined Cu-LDHs were characterized by powder X-ray diffraction and N 2 adsorption-desorption. The uncalcined solids showed clear hydrotalcite-like crystalline phases having a particle measurement between 5 and 16 nm. The best structure is attributed to the sample Cu 0.05 -Al 0.15 -LDHs. The particular surface areas are ranging between 40 and 92 m 2 /g, while the calcined samples showed the formation of Cu and Mg oxides. The antibacterial activity of Cu-LDHs with various molar ratios Cu/Al and their calcined phases were estimated towards multiple types of bacteria (Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, and Bacillus Subtilis). The Cu 0.10 -Al 0.10 -LDHs sample shows high activity against all types of bacteria either for calcined or uncalcined materials. The obtained results of the application of Cu-LDHs antibacterial inhibitors seem to be quite promising material in the antibacterial fields.

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