An experiment evaluated the ileal apparent and standardized AA and apparent energy digestibilities in grower-finisher pigs of 5 sources of distillers dried grains with solubles (DDGS) from corn. The 5 DDGS sources were analyzed for AA, GE, NDF, ADF, and color score. Diets were formulated to contain 15% CP from the test DDGS sources (approximately 60% of the diet). A low-protein (5% casein) diet was used to estimate basal endogenous AA losses. The experiment was conducted in 2 replicates of a 6 x 6 Latin Square design, with 6 treatments and six 1-wk periods. The experiment used 12 crossbred barrows [(Yorkshire x Landrace) x Duroc], averaging 28 kg of BW and 60 d of age, and surgically fitted with a T-cannula in the distal ileum. After a 10-d recovery period, treatment diets were fed in meal form, initially at 0.09 kg . BW(0.75). Feed intake was equalized between pigs within each period and increased for each subsequent period. Periods included 5 d of diet acclimation followed by two 12-h ileal digesta collections, one on d 6 and one on d 7. Apparent and standardized digestibility of AA was calculated using chromic oxide (0.4%) as an indigestible marker. The results demonstrated that apparent and standardized lysine digestibilities ranged from 24.6 to 52.3% and 38.2 to 61.5%, respectively. Average apparent essential AA digestibility was lower (P < 0.05) in sources 1 and 5, the 2 sources that were darkest in color. Apparent and standardized digestibility of the averaged nonessential AA were lower (P < 0.05) in source 5 than in all other sources. Source 5, the darkest colored DDGS, had a 10% lower (P < 0.05) average apparent and standardized essential AA digestibility and was more than 15% lower (P < 0.05) in lysine digestibility than the 3 lightest colored sources. Apparent ileal energy digestibility did not differ among the 5 sources. Lysine content and digestibility seemed to be reduced to a greater extent by the darker colored DDGS than the other essential AA, suggesting that the Maillard reaction reduced total lysine content and lowered its digestibility. These results, therefore, imply that darker colored DDGS sources may have lower (P < 0.05) analyzed lysine contents, as well as lower (P < 0.05) lysine and essential AA digestibilities, than lighter colored DDGS sources.
Five sources of corn distillers dried grains with solubles (DDGS), which varied in darkness of color, were collected from several processing plants in the Midwestern United States. Sources of DDGS were analyzed for their amino acid and energy contents, measured for color score, and evaluated for TMEn, apparent amino acid digestibility, and true amino acid digestibility. A precision-fed rooster assay was used, in which each DDGS sample was tube fed (25 g) to adult cecectomized roosters, and the excreta were collected for 48 h. The experiment was conducted as a randomized complete block design with 8 replicates. Seven adult roosters (averaging 75 wk of age) were used in each period, with 5 fed the DDGS sources and 2 fasted to estimate basal endogenous amino acid losses. One source (no. 5) was the darkest, 2 sources (no. 2 and 4) were light, whereas 2 other sources (no. 1 and 3) were intermediate in color as measured by a colorimeter. Total lysine content of the DDGS sources ranged from 0.48 to 0.76%, with the lowest lysine content in the darkest DDGS source. Apparent and true lysine digestibility was approximately 30 and 15 percentage units lower (P < 0.05), respectively, in the dark-colored source (no. 5) than in the other 4 sources. Average apparent and true digestibility of the essential amino acids were 10 and 8 percentage units lower (P < 0.05), respectively, in source 5 than the other 4 sources. The TMEn content of the 5 DDGS sources was also lower (P < 0.05) in the darkest DDGS (no. 5). Our results suggest that when the color score of a DDGS source, as measured by a colorimeter, reached a certain threshold (lightness between 28 and 34), amino acid availability and true metabolizable energy content may be reduced. This reduction was particularly evident for lysine, which had the lowest digestibility in the darkest DDGS source. These results suggest that dark-colored DDGS may have been overheated during drying, causing Maillard reactions to be more extensive and resulting in a lowered total lysine content, lysine digestibility, and TMEn content.
Four experiments involving 1,005 crossbred pigs weaned at 19 +/- 2 d of age evaluated the effect of diet complexity and lactose level on starter pig performances. Experiment 1 was a randomized complete block (RCB) conducted in nine replicates with 135 pigs. A complex diet using several protein sources, a semicomplex diet with fewer protein sources, and a simple diet of corn and soybean meal comprised the three treatment groups. All diets contained 25% lactose (as-fed basis) with lysine (total) constant from d 0 to 14 (1.55%) and d 14 to 28 (1.45%), respectively. Gain, feed intake, and feed efficiency (P < 0.05) improved as diet complexity increased during both periods. In Exp. 2, 240 pigs in eight replicates in a RCB design were fed complex diets, but dietary lactose (total; as-fed basis) levels ranged from 10 to 35% in 5% increments from 0 to 14 d after weaning. From 14 to 30 d, a common 17% lactose diet was fed to evaluate the effects of early lactose level on subsequent responses. Gains (P < 0.05) increased for the 0- to 7- and 0- to 14-d periods as lactose increased to 30%. Similar gains resulted for all treatment groups from 14 to 30 d after weaning, with no evidence of compensatory responses to early lactose levels. In Exp. 3, 330 pigs were fed complex diets. From 0 to 7 d after weaning, the diets contained 25% lactose (as-fed basis), and from 7 to 21 d postweaning, the lactose levels ranged from 7 to 31% in 5% increments. Gain (P < 0.01) and feed efficiency (P < 0.05) increased from 7 to 21 d to the 17% lactose level. In Exp. 4, 300 pigs were fed 25 and 17% (as-fed basis) lactose diets from 0 to 7 and 7 to 21 d postweaning, respectively. From 21 to 35 d postweaning, lactose levels of 0 to 20% in 5% increments were added to a corn-soybean meal diet. The experiment was conducted as a RCB design in 12 replicates. Gain (P < 0.05) and feed intake (P < 0.05) increased to 10 to 15% lactose. When the data from Exp. 4 were partitioned into lighter (15.0 kg) and heavier (17.7 kg) pig weight replicates, only the lighter replicates had significant improvements in gain, feed intake, and feed efficiency (P < 0.05) in response to dietary lactose. These results demonstrated that starter pigs performed better when fed complex diets, that dietary lactose levels of 25 to 30% (to 7 kg BW) during the initial week postweaning, 15 to 20% lactose during d 7 to 21 (to 12.5 kg BW), and 10 to 15% lactose during d 21 to 35 postweaning (to 25 kg BW) resulted in maximum performance.
Barrows and gilts of 2 genetic lines with differing lean gain potentials (high-lean = 375 g of fat-free lean/d; low-lean = 280 g of fat-free lean/d) were used to determine tissue and organ weights and compositions from 20 to 125 kg of BW. The experiment was a 2 (genetic line) x 2 (sex) x 5 (BW) factorial arrangement of treatments in a completely randomized design conducted with 2 groups of pigs in 6 replicates (n = 120 pigs). Six pigs from each sex and genetic line were slaughtered at 20 kg of BW and at 25 kg of BW intervals to 125 kg of BW. At slaughter, the internal tissues and organs were weighed. Loin and ham muscles were dissected from the carcass and trimmed of skin and external fat, and the ham was deboned. Residuals from the loin and ham were combined with the remaining carcass. Body components were ground, and their compositions were determined. The results demonstrated that tissue weights increased (P < 0.01) as BW increased. Loin and ham muscle weights increased but at a greater rate in the high-lean line and in gilts resulting in genetic line x BW and sex x BW interactions (P < 0.01). Liver and heart expressed on a BW or a percentage of empty BW basis increased at a greater rate in the high-lean line resulting in a genetic line x BW interaction (P < 0.01). Liver and intestinal tract weights were heavier in barrows than in gilts, significant only at 45 (P < 0.05), 75 (P < 0.01), and 100 (P < 0.05) kg of BW. Loin and ham muscles from the high-lean genetic line and gilts had greater (P < 0.01) water, protein, and ash contents compared with the low-lean genetic line and barrows resulting in genetic line x BW and sex x BW interactions (P < 0.01). The remaining carcass (minus loin and ham muscles) had greater (P < 0.01) amounts of water and protein, and less (P < 0.01) fat in the high-lean genetic line and gilts. The high-lean genetic line and gilts had more total body water, protein, and ash, but less body fat, with these differences diverging as BW increased, resulting in a genetic line x BW interaction (P < 0.01). The results indicated that liver and heart weights were greater in high-lean pigs, reflecting the greater amino acid metabolism, whereas the liver and intestinal tract weights were greater in barrow than gilts, reflecting their greater feed intakes and metabolism of total nutrients consumed.
A sow study evaluated the effects of 2 dietary micromineral sources (organic or inorganic) and 3 dietary mineral levels [NRC, industry (IND), and IND + Ca:P] with selected sows killed at parities 1, 2, 4, and 6. Three sows per treatment group were killed at weaning (total = 68), and their body and liver, 72 colostrum and milk samples (17 d), 69 full-term stillborn pigs and their livers, and 32 pigs at weaning were analyzed for minerals. Tissue and milk samples from the sows were analyzed as a 2 x 3 x 4 factorial arrangement of treatments in a completely randomized design (CRD) with 3 replicates per treatment. Full-term stillborn pig mineral compositions were determined at parities 1, 3, and 5 and evaluated as a 2 x 3 x 3 factorial arrangement of treatments in a CRD with 3 replicates per treatment. Weanling pigs from parity 6 sows were analyzed as a 2 x 3 factorial in a CRD. Sow and pig mineral compositions are reported on an equivalent empty BW and kilograms of liver weight basis. The results indicated that sow body macromineral contents were not affected by dietary micromineral source or level or when the diets contained added Ca and P. Sow body Se increased when dietary organic microminerals increased from the NRC to the IND level, resulting in a source x level interaction (P < 0.01), but there was no increase in those sows fed inorganic microminerals. There were increases in Cu (P < 0.05) and Se as levels increased from NRC to the IND, and there were increases (P < 0.05) in Cu and Zn when the IND + Ca:P diet was fed compared with feeding the IND diet. Increases (P < 0.01) in sow liver Cu, Se, and Zn occurred as microminerals increased from the NRC to the IND level. As parity advanced, there were cubic increases (P< 0.01) in sow body Cu, Fe, and Se, but a quadratic increase in Zn (P < 0.05). There was no clear effect of sow dietary treatments on full-term stillborn pig or liver micromineral contents, except Se (P < 0.01). There was a greater pig body Se content when sows were fed organic microminerals at the greater level, resulting in a source x level interaction (P < 0.01). Colostrum minerals were generally not affected by diet variables, except Se. Colostrum Se was greater when sows were fed the organic micromineral source than the inorganic source at the greater level, resulting in a source x level interaction (P < 0.05). Milk Cu (P < 0.01) and Zn (P < 0.01) increased as dietary level increased. Milk Se was increased when organic Se was fed (P < 0.05) and when the micromineral level was increased (P < 0.01). Weaned pig body Fe (P < 0.01) and Se (P < 0.01) were greater when organic microminerals were fed to the sow, whereas Mn (P < 0.01) and Zn (P < 0.05) increased when the IND level was fed. These results indicate that the dietary micromineral source and level had a minimal effect on sow body and liver mineral contents or in colostrum and pigs at birth, except Se, which was greater when the organic form was fed.
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