Balance studies were conducted with intact and caecectomised hens to assess the influence of the caeca on the recovery of intact glucosinolates (IG) in excreta. The hydrolysis of IG in in vitro incubations with gastrointestinal tract contents and with excreta from intact and caecectomised hens was measured to aid in the interpretation of the IG balance data. Decomposition of IG in excreta was shown to result in low recovery of IG in balance trials involving long (22)(23)(24) h) collection periods for excreta; however, balance trials with caecectomised hens and in which excreta was collected within 2 h showed high recovery. In vitro incubation studies confirmed the caeca as the major source of the IG hydrolytic activity. Free thiocyanate ion concentration of excreta of intact hens markedly exceeded that of the diet, but hydrolysis of glucosinalbin did not appear to be the major source of the thiocyanate ion.

Wheat and soybean diets supplemented with either tallow or sunflower oil (SFO) were fed to broiler chicks. Variables examined included performance, incidence of sudden death syndrome (SDS), and cardiac sarcoplasmic reticular (SR) calcium transport. The phospholipid content of heart tissues was also determined. Birds fed the SFO diet gained significantly (P < .05) more weight over the first 21 days of age and had a significantly better feed:gain ratio (P < .01). The incidence of SDS mortality up to 39 days of age was also lower (P < .05) for SFO-fed birds than for those fed the tallow diet. Calcium (45Ca2+) uptake and calcium-magnesium 5'-adenosinetriphosphatase (Ca2+ + Mg(2+)-ATPase) activity in cardiac SR vesicles did not differ due to diet (P > .05). However, compared with similar weight pen-mates showing no disease signs, SDS birds had depressed 45Ca2+ uptake (P < .01) and Ca2+ + Mg(2+)-ATPase (P < .05) of cardiac SR vesicles. The phosphatidylcholine concentration in the cell membranes of heart tissue of tallow-fed birds was significantly higher (P < .05) than in SFO-fed chicks. No differences were seen in other phospholipid constituents. The SDS birds, however, had significantly (P < .05) lower phosphatidylethanolamine plus phosphatidylglycerol, sphingomyelin, and total phospholipid concentrations in the heart tissues than the pen-mate controls. The results support the hypotheses that SDS in broilers is a cardiac dysfunction associated with defective cardiac SR membrane function and that dietary fat type is implicated with the syndrome.

No abstract

Oxidized copper wire, commonly referred to as copper oxide needles (CuON), was evaluated using purebred Hereford cows and their calves. Thirty-seven cows were allocated to Cu treatments of 0, 25 or 50 g CuON (79.9% Cu in CuON) with 12, 12 and 13 cows per treatment, respectively; calves within cow treatments were allocated to treatment of 0 and 20 g CuON. Single oral doses of CuON were given at the start of a grazing trial that lasted 92 d. Cows and calves were weighed and blood samples were taken on d 0, 28, 63 and 92; liver biopsies were taken on d 0, 28 and 92 of the grazing trial. Cattle were consuming grass forage with mean concentrations on d 0, 28, 63 and 92 of the grazing trial ranging from 1.6 to 5.5 mg/kg DM for Cu, 2.5 to 5.5 mg/kg DM for Mo and 1.3 to 1.5 g/kg DM for total S. The water consumed by cattle contained 947 mg sulfate per liter (SE = 13.2, n = 4). Body weight of cows and calves was not influenced (P greater than .05) by CuON. Liver Cu was higher (P less than .01) in treated cows and calves but was not different (P greater than .05) between cows dosed with 25 or 50 g CuON. Treatment of cows and calves with CuON had no influence (P greater than .05) on the concentration of Fe or Mo in liver or plasma, the concentration of Cu and ceruloplasmin activity in plasma, or the concentration of Zn in liver. Plasma Zn did not differ (P greater than .05) in cows, but it was higher (P less than .05) in the calves suckling cows treated with CuON. It was concluded that dosing cows and calves with CuON resulted in a higher Cu content of liver but did not adversely influence the metabolism of Fe or Zn or modify the concentration of Mo in the plasma or liver of cows or calves.

WrrrENsEnc, K. 1979