No abstract

No abstract

We studied the polyphenol compositions of the plantains Plantago major L. and P. lanceolata L. in order to find new sources of drug raw material. For this, the total polyphenols were isolated from the aerial parts of the plants (yields of 4.6% and 5.4, respectively, of the air-dried raw material mass). Paper chromatography using system 1 (n-BuOH:AcOH:H 2 O, 40:12:28) found that polyphenols from P. major contained 11 compounds. Column chromatography over hide powder using Et 2 O (1), H 2 O (2), and aqueous acetone (60%) (3) separated the total polyphenols into three fractions [1].The first fraction contained a single compound (compound 1), R f 0.51 (system 2, n-BuOH:AcOH:H 2 O, 4:1:5, upper phase). The second fraction had five compounds with R f 0.45, 0.58, 0.53, 0.35, and 0.64 (system 3, n-BuOH:AcOH:H 2 O, 4:1:2). Rechromatography of it over a column of polyamide with elution by aqueous EtOH of various concentrations separated the pure compounds (compounds 2-6). The third fraction was rechromatographed over a column of silica gel with elution by a gradient of Et 2 O:EtOAc with increasing EtOAc content to isolate five pure compounds with R f 0.68, 0.36, 0.31, 0.22, and 0.14 (system 1) that were tannins (compounds 7-11).Compounds 1-9 were identified from the physicochemical properties as gallic acid (1), quercetin-3-rutinoside (rutin) (2), 5,7,3c,4c-tetrahydroxyflavone (luteolin) (3), 3,5,7,4c-tetrahydroxy-3c-methoxyflavone (isorhamnetin) (4), quercetin-3-O-E-D-galactopyranoside (hyperoside) (5), 3,5,7,3c,4c-pentahydroxyflavone (quercetin) (6) [2, 3], 1,2,3,4,6-penta-O-galloyl-E-D-glucose (7), 1,2,3-tri-O-galloyl-E-D-glucose (8), and 1,3,4,6-tetra-O-galloyl-E-D-glucose (9) [2, 3]. Hexahydroxydiphenoyl-1-(O-2-O-galloyl-E-D-glucopyranoside)-1-(O-E-D-xylopyranoside) Diester (10).C 32 H 32 O 23 , white amorphous powder, R f 0.22 (system 1). The acid hydrolysis products (HCl, 5%) were xylose, glucose, gallic and ellagic acids. UV spectrum (EtOH, O max , nm): 265, 283. IR spectrum (KBr, Q, cm -1 ): 3350-3345 (OH), 1730-1710 (ester), 1620-1510 (Ar), 1020-1010 (sugar). PMR spectrum (200 MHz, acetone-d 6 , G, ppm, J/Hz): 4.24 (1H, d, J = 7.7, xyl-1), 3.03 (1H, m, J = 9.3, xyl-2), 3.11 (1H, m, J = 6.2, xyl-3), 3.26 (1H, m, J = 9.3, xyl-4), 3.63 (1H, m, J = 8.5, xyl-5), 4.01 (1H, t, J = 8, Glu

No abstract

The aim of this work is to study the composition and structural characteristics of the polyphenols of the tanning sumac Rhus coriaria L. of the Anacardiaceae family, growing in Uzbekistan, using a high-performance liquid chromatograph with a diode-matrix detector (HPLC-DAD) and a tandem temple mass-spectrometer (HPLC– Q-TOF-MS / MS). The phenolic compounds of the aerial part (leaves) of Rhus coriaria L. plants of the Anacardiaceae family were studied. For the first time, polyphenol fractions were isolated from tanning sumac leaves using stepwise hydrophobic chromatography. By HPLC, as a result of semi-preparative chromatography from the fraction eluted with 30% ethanol, 9 individual compounds were obtained, in the amount of: R-1 – 0.5 mg, R-2 – 0.8 mg, R-3 – 2.3 mg, R-4 – 12.6 mg, R-5 – 34.5 mg, R-6 – 15 mg, R-7 – 8 mg, R-8 – 7.1 mg, R-9 – 45.5 mg As a result of mass spectrometric analyzes and NMR spectroscopy for individual polyphenols, it was established that the polyphenols isolated in the individual state consist of gallic acid and glucose, interconnected by an ester bond: mono-, di-, tri-, tetra-, penta- , hexa-, hepta-, octa- and non-O-galloyl-β-D-glucose.