No abstract

The scientific article presents the results of research on the development of an effective method for isolating nucleic acids by citrate-salt hydrolysis from the freeze-dried biomass of Chlorella vulgaris microalgae to create an oral preparation based on them in the form of a sodium salt of nucleic acids, as well as determining its effect on morphological and biochemical parameters of rabbit blood, in particular protein metabolism. A special bioreactor was developed and constructed for the experiment. The author's method of extracting nucleic acids from the biomass of green microalgae of the genus Chlorella vulgaris was developed and tested to obtain the final product in the form of sodium nucleinate powder with the necessary physico-chemical parameters, which was determined by appropriate analysis methods. The yield of sodium nucleinate after drying in an air current and weighing on an electronic scale with an error of ± 0.0002 g in mass ratios was 6.0 g from a sample of 50.0 g of dry biomass of Chlorella vulgaris microalgae. The resulting preparation is a white - beige finely dispersed amorphous powder, odorless after the stages of washing and drying. Electrophoretic analysis determined that the preparation of nucleic acids from chlorella has a molecular weight of 350 to 400 kDa. It has been established that the obtained preparation based on nucleic acids has a positive effect on morphological and biochemical parameters of blood and indicators of protein metabolism of rabbits. The increase in the content of total protein, albumins, gamma globulins in rabbits receiving the drug was more pronounced compared to the control group of rabbits. An increase in the level of gamma globulins in rabbits treated with a nucleic preparation indicates the activation of humoral protection factors in them.