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The field experiments were conducted at ICAR Research Complex for North Eastern Hill Region, Umiam, Meghalaya, India during 2011-2012 to evaluate some biopesticides and newly introduced insecticides against shoot and fruit borer of brinjal. The experiments were laid out in Randomized Block Design (RBD) with three replications. The treatments viz., azadirachtin 1 EC (2 ml l-1), karanjin 2 EC (2 ml l-1), anonin 1 EC (2 ml l-1), Bacillus thuringiensis (Bt.) (2 g l-1), emamectin benzoate 5 SG (0.4 ml l-1), flubendiamide 480 SC (0.3 ml l-1), chlorantraniliprole 18.5 SC (0.4 ml l-1), chlorpyriphos 20 EC (2 ml l-1) were applied thrice at fifteen days interval starting from initiation of shoot and fruit borer infestation. Results showed that chlorantraniliprole (2.46%) was the best treatment in reducing the shoot infestation (81.88% reduction) which was statistically at par with flubendiamide (3.08%) and emamectin benzoate (3.76%) with 77.37% and 71.95% reduction over untreated control, respectively. The overall mean fruit infestation were also minimum in chlorantraniliprole (5.76%) and flubendiamide (5.93%) treated plots with 79.45% and 78.84% reduction over untreated control plots (28.03%), respectively. Pooled data of two years experimental results indicated that highest marketable yield was recorded in chlorantraniliprole treated plots (155.01 q ha-1) followed by flubendiamide (149.50 q ha-1), emamectin benzoate (134.24 q ha-1) and chlorpyriphos (125.43 q ha-1). Among bio-pesticides, highest mean marketable yield was recorded in Bacillus thuringiensis treated plots (114.45 q ha-1) followed by azadirachtin (101.11 q ha-1).

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Ladybird beetles are generally considered as agriculturally beneficial insects, but the ladybird beetles in the coleopteran subfamily Epilachninae are phytophagous and major plant feeding pest species which causes severe economic losses to cucurbitaceous and solanaceous crops. Henosepilachna pusillanima (Mulsant) is one of the important pest species of ladybird beetle. In this report, we sequenced and characterized the complete mitochondrial genome of H. pusillanima. For sequencing of the complete mitochondrial genome, we used the Ion Torrent sequencing platform. The complete circular mitochondrial genome of the H. pusillanima was determined to be 16,216 bp long. There were totally 13 protein coding genes, 22 transfer RNA, 2 ribosomal RNA and a control (A + T-rich) region estimated to be 1690 bp. The gene arrangement and orientations of assembled mitogenome were identical to the reported predatory ladybird beetle Coccinella septempunctata L. This is the first completely sequenced coleopteran mitochondrial genome from the beetle subfamily Epilachninae from India. Data generated in this study will benefit future comparative genomics studies for understanding the evolutionary relationships between predatory and phytophagous coccinellid beetles.

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