No abstract

The cerebellum is a complexly organized multifunctional component of the brain and a target in numerous lesions, so the study of its morphofunctional state in various pathological conditions and burns does not lose its relevance. The aim of our study was to establish the features of micro- and submicroscopic changes in the structural components of the cerebellar cortex after 21 days under conditions of experimental thermal trauma. The experimental study was simulated on white laboratory male rats. Grade III burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the affected area was 18-20% of the epilated surface of the body of rats. Histological changes were studied for 21 days from the beginning of the experiment. Semi-thin sections for light microscopy were stained with methylene blue, for electron microscopy the obtained ultra-thin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. With the help of micro- and submicroscopic studies, significant alternative changes of both neurons of all layers of the cerebellar cortex and parts of the microcirculatory tract of the organ were revealed. Neurons are disorganized, their shape is changed, there is low functional activity, due to a decrease in the area of the chromatophilic substance. Often there are cell-free areas in the ganglion layer, there is a shift of Purkinje cells deep into the granular layer, and grain cells are pushed sharply into the higher molecular layer. Thus, 21 days after the experimental thermal injury, destructive-degenerative changes of neurons of the cerebellar cortex, paravasal edema and disorders of transendothelial metabolism were established.

The work is a fragment of scientific research "Peculiarities of structural reorganization of the nervous, digestive, endocrine systems, hematopoietic and immune organs under conditions of thermal injury and the use of corrective factors" (0120U104152).The study of the state of internal organs and systems of the body under the influence of external factors that are stressful is relevant. The negative impact of environmental factors on the central nervous system, including cerebellum, leads to their structural and functional restructuring. The cerebellum is a multifunctional component of the brain and the object of numerous lesions.The purpose of this work is analysis of scientific literature which is dedicated study of the influence of exogenous and endogenous factors on the morphological structure of the cerebellum.The article analyzes the scientific literature of the effects of hypodynamia, hypothermia, hypokinesia, alcohol intoxication, toxic effects of Lead, drugs, pharmacological

No abstract

Thermal injury is a lesion caused by thermal agents. With deep and large burns, the course of this type of injury becomes a general disease of the whole organism with the involvement of vital organs. The skin is the first to perceive the action of this exogenous factor, and secondarily there are changes in all body systems, in particular in the cerebellum. The aim of our study was to establish micro- and submicroscopic changes in the structural components of the cerebellar cortex one day after the experimental thermal injury. Simulation of experimental thermal injury was performed on white laboratory male rats. Grade III burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the affected area was 18-20 % of the epilated body surface of rats. The cerebellum was collected after one day, further processing of the material for micro- and submicroscopic examination was performed according to accepted methods. Histological specimens were stained with methylene blue, and for electron microscopy the obtained ultrathin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. At the micro- and submicroscopic levels, it was found that 1 day after the experimental thermal injury, reactive changes are observed in neurons, microcirculatory tract and glial cells. Thus, one day after the thermal trauma of the skin, the experimental animals showed the initial violations of the blood-brain barrier of the cerebellar cortex, which are adaptive-compensatory in nature. Initial, destructive, changes in neuro-glial-capillary relations, which occur primarily on their damage to the walls of hemocapillaries and are manifested by a violation of the micro- and ultrastructure of the main cells of the cerebellum – Purkinje, neurocytes of molecular and granular layers, neuroglyocytes, with peri-gliocyocytes were found.