Medicinal species of cork trees Phellodendron amurense Rupr., P. chinease Sohneid, P. wilsonii Hayate et Kemachica, and P. amurense Rupr. var. sachalinense Fr. (Rutaceae) are distributed in the Far East, China, and Korea. They all are productive sources of the protoberberine alkaloids berberine, palmatine, jatrorrhizine, and phellodendrine. Cork phloem, leaves, and fruit are used for medicinal purposes in Eastern medicine. Decoctions and tinctures of cork trees exhibit antimicrobial, analgesic, anti-inflammatory, antidiabetic, and other activities [1-6].The Lavalle cork species that was introduced to Western Georgia is insufficiently studied. The content of berberine in it has been reported [7].Pharmacological in vitro tests of the dry aqueous extract of bark from this plant for anti-inflammatory, antimicrobial, and cytotoxic activity at the Basic Research Department of Chicoutimi University (Canada) showed pronounced antiinflammatory activity.Pharmacological in vivo tests at the Pharmacology Department of Tbilisi State Medical University found hypoglycemic activity for the dry aqueous extract of Lavalle cork. This prompted our studies of the alkaloid composition in Lavalle cork bark collected in 2008-2009 on the shores of the Adzhari Black Sea.The aqueous extract of cork bark was studied. Purification of the extract from polypeptides, resins, and water-soluble polysaccharides; distribution according to solubility between butanol and the aqueous phase; and separation over a column of silica gel isolated the hydrochlorides and iodide of four constituents (1-4), of which 1 and 4 dominated and 2 and 3 were minor compounds.Analysis of DEPT, PMR, and 13 C NMR spectral data of 1 and 2 (Table 1) in addition to the TLC mobility, the lack of melting point depression of samples of these alkaloids and standard berberine and jatrorrhizine samples, and a comparison with the literature enabled 1 and 2 to be identified as berberine and jatrorrhizine, respectively [5,8,9,10].Mass spectrum of 3 (EI, 12 eV, m/z):
