A previously healthy 50-year-old man was transferred to our hospital for evaluation of acute inflammation in his right eye after ocular trauma while using a grass mower. Slit lamp examination showed 1 mm-length full thickness corneal laceration without leakage, 4+ cells and inflammatory membrane in the anterior chamber, 10% hypopyon, posterior synechiae formation, and cataract change. Upon orbital computerized tomography, a metallic intraocular foreign body in the lens was indentified. Vitrectomy, phacoemulsification, foreign body removal, anterior chamber irrigation, and intravitreal antibiotics injections of vancomycin and ceftazidime were performed. In a culture of humor from the anterior chamber grew Pantoea species. More procedures were performed, including intravitreal antibiotics injection of ceftazidime. Upon administering a course of intravenous ceftazidime, fortified ceftazidime and moxifloxacin eye drops, and oral prednisolone, the patient improved.

Purpose:To evaluate the clinical characteristics of bacterial culture, and visual outcome in patients with acute endophthalmitis. Methods: Clinical records of patients treated for acute endophthalmitis in GNUH from 2000 to 2009 were reviewed. The specimens for culture were obtained from the anterior chamber or vitreous. Clinical outcome measures were bacterial culture, culture rate, and final visual acuity. Results: Cultures (total 59 cases) showed bacterial growth in 37 cases (63%). Among 35 cases vitreous specimens, bacteria growth was found in 22 cases (63%), and from the 27 anterior chamber specimens, 12 cases (44%) were culture positive. From these 37 bacterial-positive cultures, 11 (30%) were coagulase negative Staphylococcus species, 16 (43.0%) were other Gram-positive species, 9 (24%) were Gram-negative species, and 1 (3%) produced a polymicrobial culture. Final visual acuity above 0.5 was achieved in 16 of 59 (27%) cases and coagulase negative Staphylococcus species had the greatest proportion being 5 of 11 (45%). Conclusions:The bacterial culture positivity rate in bacterial endophthalmitis was 63%, and the culture yield rate from the vitreous was higher than the anterior chamber aqueous samples. Coagulase negative Staphylococcus species were the most common causative organisms and showed the best final visual outcome in endophthalmitis.

PurposeTo identify altered patterns of retinal mRNA expression in a rat model of oxygen-induced retinopathy (OIR).MethodsSprague-Dawley rats from P2 to P14 were exposed to hyperoxia (80% oxygen) to induce OIR and then returned to normoxic conditions. Control rats were sustained in room air. Retinal gene expression between the rats of OIR and the controls was compared using cDNA microarray analysis. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to verify the microarray results.ResultsAmong a total of 12,731 cDNAs analyzed by mircroarray, 13 genes were strongly up- or down-regulated (>2-fold change over controls) in the OIR rats. We found a significant increase in expression of 10 genes (CaM-kinase II inhibitor; acidic nuclear phosphoprotein 32 family, member A; vascular endothelial growth factor; interferon α-inducible protein 27-like; similar to enthoprotin, epsin 4, clathrin interacting protein; nidogen [entactin]; tubulin, β5; fibrillin-1; spectrin β2; and stearoyl-coenzyme A desaturase 2) and a significant decrease in expression of 3 genes (myelin-associated oligodendrocytic basic protein, heat shock protein, and decorin) in OIR rats compared to controls.ConclusionsWe confirmed changes in expressions of various retinal genes in a rat model of OIR by microarray and RT-PCR. This study should contribute to the understanding of genetic indicators associeated with OIR.

No abstract

No abstract