Background: Immunization is when a child is made immune or resistant to an infectious disease by administering a vaccine. These vaccines help to stimulate the body's immune system to protect the children against subsequent diseases. This study aims to evaluates effect of health education for Mothers' of Children Under-Five-Year regarding Immunization in Rural Area. Design: The quasi-experimental test design was employed. Sample: A purposive sample included 70 mothers had children with age under five years and more attended the routine immunization. Setting: The research was carried out at the Sanors district of El-Fayoum City's Maternal-Child Health Centers (MCH) (rural area). Two tools for data collection: Tool I Demographic structured interview sheet Tool 11: Mother knowledge and practices about immunization of children under five years (pre-post format). Results: the mother's knowledge and practices increased after applying health education, Post-health education mothers had 75.7% good experience than (28.6%) pre-health education for mothers. In addition, found significant between pre-health education and post for reported immunization practices. Conclusion: The study found that mothers' knowledge and practices improved more in the post-health education than in the pre-health education phase, with a statistically significant difference. Recommendations: In MCH center, mothers should be provided with health information and immunization booklets that the benefits of immunization, side effects, home care, obligate vaccination plans, and a non-obligatory vaccine schedule.