Introduction:In this study, we used dichloromethane (DCM) and methanol (MeOH) extracts of the Zingiber zerumbet rhizome to evaluate brine shrimp lethality and larvicidal activity on Aedes aegypti and Anopheles nuneztovari mosquitoes. Methods: Bioassays were performed by exposing third-instar larvae of each mosquito species to the DCM or MeOH extracts. Results: Probit analysis with DCM and MeOH extracts demonstrated effi cient larvicidal activity against A. aegypti and A. nuneztovari larvae. Conclusions: The DCM and MeOH extracts showed higher activity against A. nuneztovari larvae than against A. aegypti larvae, suggesting that the extracts have species-specifi c activity.