Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith

Bücker, Augusto; Falcão-Bücker, Nádia Cristina; Núñez, Cecília Veronica; Pinheiro, Carlos Cleomir de Souza; Tadei, Wanderli Pedro

doi:10.1590/0037-8682-1309-2013

Cited by 14 publications

(5 citation statements)

References 12 publications

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“…The lytic effects of Zerumbone were estimated according to Ghramh et al (7) with minor modifications. Three mL of human blood was withdrawn using anticoagulant agent EDTA (10) in compliance with Compound dissolved in 100% DMSO and then diluted in PBS for experiment, from here, 10, 5, 2.5 µL was picked up to make 1000, 500 and 250 µg/mL, respectively.…”

Section: Hemocompatibility Assay For Zerumbonementioning

confidence: 99%

“…Zerumbone is a major bioactive compound derived from the rhizomes of Zingiber zerumbet (Smith) that has been traditionally used for the treatment of stomach ulcer, diarrhea and anti-inflammatory (5,6). The methanolic and dichloromethane extract of rhizomes of Z. zerumbet had been reported to possess anti-larvicidal property (7), whereas the ethyl acetate extract of this plant was reported for providing protection to erythrocytes from the oxidative damage induced by hydrogen peroxide (8). In these reported manuscript, only extracts were tested, and predicted zerumbone as the possible compound responsible for these activities.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity

2021

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Zerumbone is a well-known compound having anti-cancer, anti-ulcer, anti-inflammatory and anti-hyperglycemic effects. During its use for the disease treatment, the membrane of erythrocyte can be affected by consumption of this bioactive compound. The current study was the first report of investigation of the hemolytic activities on human erythrocytes and cytotoxic profile of zerumbone. The toxicity of zerumbone on human erythrocytes was determined by in vitro hemolytic assay. Brine shrimp lethality assay was used to evaluate the cytotoxic effect of zerumbone at concentrations 10, 100 and 1000 μg/mL. The human erythrocyte test showed no significant toxicity at low concentrations, whereas hemolytic effect was amplified up to 17.5 % at the highest concentration. The half lethal concentration (LC50) value of zerumbone against brine shrimp was less than 1000 µg /mL (LC50=190 µg/ml) showing the significant toxic nature of this compound. These results provide a baseline in terms of the toxicity of therapeutic formulations from this compound to membrane erythrocytes with a great attention to the highest concentrations, which paves promise for drug development.

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Section: Hemocompatibility Assay For Zerumbonementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity

2021

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“…Six replicates were maintained at a time for each experiment. The LC50 was calculated using GraphPad prism 5 software (Bucker et al, 2013).…”

Section: Larvicidal Activitymentioning

confidence: 99%

Larvicidal, leishmanicidal, insecticidal and anthelmintic effects of Sterculia diversifolia stem bark and leaf

Rabbi

Zada

Nisar

2020

Bangladesh J Pharmacol

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The present study was conducted to evaluate the stem bark and leaf of Sterculia diversifolia for larvicidal, leishmanicidal, insecticidal and anthelmintic activities. In the larvicidal activity, dichloromethane and ethyl acetate fractions of both stem bark and leaf showed significant results, while the ethyl acetate fraction showed mild activity (IC50: 70.09 ± 0.09 μg/mL; 71.25 ± 0.01 μg/mL). In the insecticidal activity of the stem bark, n-hexane fraction showed mild activity (20-40% mortality) against all three subjects, while the insecticidal activity of leaf, methanolic extract and n-hexane fractions showed moderate activity (60% mortality) against Rhyzopertha dominica and Callosobruchus analis. In the anthelmintic activity of stem bark and leaf, ethyl acetate fraction exhibited activity in a dose-dependent manner giving time of paralysis and death shorter followed by dichloromethane fraction with 200 mg/mL concentration. Hence, it is clear that S. diversifolia stem bark and leaf possess larvicidal, leishmanicidal, insecticidal and anthelmintic agents. Video Clip of Methodology: Anthelmintic Activity: 5 min 29 sec: Full Screen Alternate

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“…A preliminary study of this species found that the rhizome of Z. zerumbet contained a variety of naturally-occurring compounds, such as terpenoids and phenolic compounds, including flavonoids, alkaloids, and saponins [5][6][7]. In addition, the dichloromethanolic extract of Z. zerumbet rhizome tested against Artemia salina Leach (Brine shrimp lethality test) resulted in 40.4 µg/mL lethal concentration (LC 50 ), while on Anopheles nuneztovari, the LC 50 reached 62.8 µg/mL [8]. These values indicate that the rhizome of Z. zerumbet has the potential to be developed into a larvicide.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Naturally Occurring Larvicidal Compound Isolated From Zingiber Zerumbet (L).J.E. Smith.

Murini

Wahyuningsih

Satoto

et al. 2018

Asian J Pharm Clin Res

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Objective: A naturally occurring larvicidal compound is an alternative to eradication of Aedes aegypti larvae. This compound is toxic to larvae but relatively safe for human. Rhizome of Lempuyang gajah (Zingiber zerumbet (L.) J. E. Smith) has been used traditionally to prevent mosquito bites. An initial study indicated that petroleum ether (PE) soluble fraction of the methanol (MeOH) extract of Z. zerumbet was toxic against larvae lethal concentration (LC 50 ), 67.01±2.35 versus 153.57±4.01 ppm (MeOH ext.). Therefore, this study aimed to isolate and identify the compounds with larvicidal activity from Z. zerumbet rhizome. Methods:The PE soluble (PE-soluble) fraction was subjected to a bioassay-guided fractionation and isolation method to obtain four (4) fractions (I-IV). Two compounds (1 and 2) were isolated from Fraction I that was the most active compared to other fractions (II-IV). Compound 1 turned out to be more active than compound 2; therefore, compound 1 was then identified by means of spectroscopic data. Results Conclusion:Zerumbone was the main active compound; in the future, this compound can be formulated as a standardized preparation based on the content.

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Evaluation of larvicidal activity and brine shrimp toxicity of rhizome extracts of Zingiber zerumbet (L.) Smith

Cited by 14 publications

References 12 publications

Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity

Evaluation of Hemolysis Activity of Zerumbone on RBCs and Brine Shrimp Toxicity

Larvicidal, leishmanicidal, insecticidal and anthelmintic effects of Sterculia diversifolia stem bark and leaf

Isolation and Identification of Naturally Occurring Larvicidal Compound Isolated From Zingiber Zerumbet (L).J.E. Smith.

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