No abstract

Thymus vulgaris L. (thyme), Origanum majorana L . (marjoram), and Origanum vulgare L . (oregano) were used to determine whether light modification (plants grown under nets with 40% shaded index or in un-shaded open field) could improve the quantity and quality of essential oils (EOs) and antioxidant activity. The yield of EOs of thyme, marjoram, and oregano obtained after 120 min of hydrodistillation was 2.32, 1.51, and 0.27 mL/100 g of plant material, respectively. At the same time under shading conditions plants synthetized more EOs (2.57, 1.68, and 0.32 mL/100 g of plant material). GC/MS and GC/FID analyses were applied for essential oils determinations. The main components of the thyme essential oil are thymol (8.05–9.35%); γ-terpinene (3.49–4.04%); p-cymene (2.80–3.60%) and caryophyllene oxide (1.54–2.15%). Marjoram main components were terpinene 4-ol (7.44–7.63%), γ-terpinene (2.82–2.86%) and linalool (2.04–2.65%) while oregano essential oil consisted of the following components: caryophyllene oxide (3.1–1.93%); germacrene D (1.17–2.0%) and (E)-caryophyllene (1.48–1.1%). The essential oil from thyme grown under shading (EC 50 value after 20 min of incubation) have shown the highest antioxidant activity – 0.85 mg mL −1 in comparison to marjoram and oregano (shaded plants EC 50 19.97 mg mL −1 and 7.02 mg mL −1 and unshaded, control plants EC 50 54.01 mg mL −1 and 7.45 mg mL −1 , respectively). The medicinal plants are a good source of natural antioxidants with potential application in the food and pharmaceutical industries. For production practice, it can be recommended to grow medicinal plants in shading conditions to achieve optimal quality parameters.

In the research carried out in the period from 2009-2012, in the north of Montenegro, the influence of cultivar and different amounts of nitrogen on the productive traits of winter triticale was examined. The research covered 5 cultivars of winter triticale (Odyssey, Kg-20, Triumph, Rtanj and Tango). The experiment was set up by random block system in four repetitions. Unfertilized plot (the control) and three steps of N fertilization (60, 90 and 120 kg ha-1 N) on the same level of phosphorus and potassium (80 kg ha-1 P 2 O 5 + 80 kg ha-1 K 2 O) were applied. The results showed that genotypes respond to the application of mineral nutrients, as well as to the increased nitrogen levels by changing productive properties. Variety Tango had the highest average grain yield, while Kg-20 had the lowest. Also, Tango had the highest value of the 1000 grain weight, while variety Triumph had the highest value of hectolitre weight. The application of fertilizers has led to a very large and significant increase of yield compared to the control. Based on the analysis of variance, it can be concluded that there were highly significant differences in grain yield among years of the research and significant differences at the 1000 grain weight and hectolitre weight.

The aim of this study was to determine the antimicrobial activity of essential oils obtained from sweet basil (Ocimum basilicum L. cv. ‘Genovese’) cultivated in the open field under different shading conditions (red, blue, and pearl nets with a shade index of 50% and full sunlight exposure (control plants)), harvested at different times. The antimicrobial activity of basil essential oils (BEOs) obtained from all samples was determined for four microorganisms, while determinations for an additional five microorganisms included samples from non-shaded plants, plants grown under red and pearl nets, and second harvest of plants grown under blue net. Basil essential oil exhibited antimicrobial activity surpassing the activity of relevant commercial antibiotics regardless of growing conditions in the case of B. cereus, K. pneumoniae and C. albicans, while superior antimicrobial activity was exhibited in the case of essential oils from plants grown under blue nets in the case of S. aureus, E. coli and P. vulgaris. The influence of the application of colored shading nets was highly significant (p < 0.01) in the cases of all analyzed microorganisms except C. albicans and P. aeruginosa, while the influence of harvest time was proven in the cases of all microorganisms except K. pneumoniae. ANOVA proved that antimicrobial activities are highly dependent on the methods of plant production, shading treatment, and harvest time. Obtained results are discussed in relation to previously determined composition and yield of essential oils from basil grown under shade nets and harvested in different periods.

The manipulation of light intensity by shade nets can lead to exchanges in the phytochemical quality and antioxidants ofsome herbs. This study aimed to determine whether shading by pearl nets (50% shade index) could improve the bioactive compounds in several medicinal herbs such as thyme (Thymus vulgaris L.), marjoram (Origanum majorana L.), oregano (Origanum vulgare L.), lemon balm (Melissa officinalis L.), and peppermint (Mentha piperita L.), and their correspondingherbal extracts during a four-week maceration process in ethanol. Oregano and thyme provided the highest yield of total extractive substances (TES) from both shaded and non-shaded plants. Among all studied herbs, the highest level of antioxidants, expressed as total phenolic content (TPC) was found in extracts from shaded plants of lemon balm. Herbal extracts produced from non-shaded thyme and marjoram had higher flavonoid contents compared to herbal extracts from shaded plants. Accumulation of the investigated secondary metabolites depends more on the specificity of a plant species rather than light intensity. This study was an initial step in the production of medicinal plants with an increased quantity of antioxidant and other bioactive compounds during the maceration process in extracts, aimed to be used as natural alcoholic productwith added value.