Background: Biological, psychological and social changes make adolescents a vulnerable group and need special attention. Optimal nutritional status is indirectly influenced by good knowledge. Meanwhile, the regularity of the menstrual cycle is considered an important factor in reproductive health. Objective: The aim of the study was to determine the relationship between knowledge and nutritional status with the menstrual cycle of female adolescents.. Design: This analytic observational study used a cross-sectional design. Sampling using a simple non-random technique. Nutrition knowledge level data obtained from nutrition knowledge questionnaire with good and poor categories. Body mass index for age (BMI-for-age)) is used to categorize nutritional status. There are two categories for the menstrual cycle's characteristics: normal and abnormal. The menstrual cycle survey was created as a Google form and administered online. Results: Respondents in this study were 88 students of SMA Negeri 1 Kroya. The study found that 55% of respondents had poor nutritional knowledge. The majority of respondents' nutritional status in this study was good nutrition (66%). Research found as many as 9% of respondents are more nutritional status. Research has found that more than 70% of respondents have abnormal menstrual cycles. This study found no relationship between knowledge and the menstrual cycle in young women and there was no relationship between nutritional status and the menstrual cycle in young women. Conclusions: Nutritional knowledge and nutritional status are not statistically related to the menstrual cycle in young women at SMAN 1 Kroya. The majority of respondents have insufficient knowledge of nutrition, so it is important to promote balanced nutrition for young women to increase their knowledge of nutrition