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Background: Diabetic foot ulcers (DFUs) are major public health problem, especially in developing countries. Still now management of diabetic foot ulcer is the major challenge for surgeons.Methods: A prospective study was done at Kamineni Institute of Medical Sciences, Narketpally; from February 2014 to January 2017. All the collected data was analyzed by the SPSS 20.0 software.Results: A total of 350 DFUs patients was included in this study. Among all of them 77.14% were male and 22.86% were female. Most common age group with DFUs was 40-60 years. All the patients had type 2 diabetes mellitus. The majority of patients were presented foot ulcers between 4 weeks to 32 weeks. 18% patients had previous history of DFUs and 11.14% had previous amputation. The forefoot was commonly affected in 48.3% of cases. Neuropathic ulcers were the most common type of DFUs in 50.6% of cases. Wagner's stage 3, 4 and 5 ulcers were the most common at 20.86%, 28% and 22% respectively. 52.86% patients were treated surgically. Lower limb amputation was the most common surgical procedure performed in 60% of cases. The complication rate was (49.7%) and surgical site infection was the most common complication (34.8%). 60% of cultures had polymicrobial growth and Staphylococcus aureus (53.3%) was the most common microorganism isolated. Most of the microorganisms isolated showed multi drug resistance to commonly used antibiotics except for Carbapenems group drugs (sensitivity 100%). The hospital stay was ranged between 15-116 days. Mortality rate was 9.4%.Conclusions: Diabetic foot ulcer is the major cause of morbidity and mortality among patients with diabetes mellitus. A proper education on foot care and appropriate foot ware, good sugar level control and early surgical intervention is needed to decrease the morbidity and mortality among diabetic foot ulcer patients.

<p class="abstract"><strong>Background:</strong> Otitis media is one of the common infection prevalent worldwide. Chronic ear infection in older children can cause hearing loss which leads difficulty in communication, learning and social adjustment. It can result to severe disability, death due to central nervous system disorder in developing countries. Hence, the present study aimed to determine the bacteriological profile and its antibiogram of ear infection.</p><p class="abstract"><strong>Methods:</strong> This study conducted in ENT department of MNR Medical College and Hospital during the period of October 2019 to January 2020.  </p><p class="abstract"><strong>Results:</strong> A total of 120 patients were included in this study. Among the total, 70 were adult (58.3%), age group ranges between 20-55 years and 50 were children (41.6%). Bacterial isolates from ear swab included; <em>Staphylococcus aureus</em> 28, <em>Pseudomonas </em>19, <em>Klebsiella spp</em> 15, <em>Proteus spp</em> 10, <em>Escherichiae coli</em> 7, <em>Enterobacter spp</em> 4 and <em>Citrobacter spp</em> 2. Overall bacteria mostly sensitive to drugs like pipercillin tazobactum (85%), amikacin (90%) and gentamycin (90.2%). Other than these drugs ampicillin (90.1%), cefazolin (90%), cefuroxime (89.4%), ceftriaxone (81%), oxacillin (83.4%), penicillin (88.2%) showed highly resistant to bacterial isolates.</p><p class="abstract"><strong>Conclusions:</strong> Therefore, it is clear from this study that, most of the prescribed drugs were resistant to bacteria isolated from ear infection. Hence it is mandatory to include culture and sensitivity report to manage the cases of otitis media.</p>

Malaria is a fatal life-threatening parasitic infection and a leading cause of morbidity and mortality. The present study was aimed to evaluate simple, inexpensive, accurate, reliable, easily available better diagnostic for rapid detection of malaria at point of care (POC). The study includes 1403 samples collected from the patients, of which 1227 were clinically suspected cases and 176 from consecutive feverish patients. Among the suspected cases only 338 samples were confirmed positive and 889 samples were negative for Plasmodium species by PCR. All the 889 samples showed negative result for plasmodium species by microscopy, Malarial Ag rapid kits but only 867 samples were confirmed negative with malarial Ab rapid kits. Of the 338 PCR positive samples, 337 samples were confirmed positive by microscopy and Malarial Ag rapid kits, but only 284 samples were confirmed positive using malarial Ab rapid kits. Overall the microscopy and the malaria antigen-based lateral flow assay exhibited similar sensitivity, specificity, PPV, NPV and efficiency, respectively, whereas the PCR assay had 100% sensitivity, specificity, PPV, NPV and efficiency. But the evolutionary data for malaria antibody lateral flow assay has 92.81% sensitivity, 94.13% specificity, 84.02% PPV, 97.52% NPV and 93.80% efficiency. The developed Malaria pf/pv antigen and antibody field-deployable kits are simple, rapid, accurate, reliable, inexpensive, user friendly, POC. In addition the kits are highly sensitive and species-specific. The pf/pv antigen kit is found to be more accurate with 99.7% sensitivity and 100% specificity than to Malaria pf/pv antibody rapid kits. Of the two rapid kits developed, Malaria pf/pv antigen kit is found be more accurate with 99.7% sensitivity and 100% specificity than to Malaria pf/pv antibody rapid kits. KeywordsPoint of care (POC) • Immuno chromatographic test (ICT) • Plasmodium Lactate Dehydrogenase (pLDH) • Histidine Rich Protein II (HRPII) • Plasmodium falciparum (pf) • Plasmodium vivax (pv) • Antigen (Ag) • Antibody (Ab) • Rapid diagnostic test (RDT) • Polymerase chain reaction (PCR)

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