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This study was conducted on 45 heads (15 of each breed) of three Sudanese domestic goat breeds (Desert goat, Nubian goat and Taggar goat) to pursue complete mitochondrial DNA analysis using PCR-RFLP method to detect the mitochondrial DNA diversity of two mit-DNA haplogroups, lineage A and lineage D. The results revealed that most individuals of the three breeds belonged to lineage A with frequencies 0.2 of Desert goats, 0.6 of Nubian goats and 0.6 of Tagar goats. Some of the individuals belonged to modified lineage A with frequencies 0.2 of Desert goats, 0.2 of Nubian goats and 0.2 of Tagar goats; no individual of each belonged to lineage D. In conclusion, the appearance of individuals that belonged to modified lineage A in the different goat breeds might be due to mutation resulted from insertion or deletion of events and might a criterion of the Sudanese indigenous goat breeds. However this suggestion needs more confirmative analysis.

The purpose of this study was to estimate relationship between and within some Sudanese camel breeds (Kababeish, Shanabla and Nyalawei) by digestion of mitochondrial DNA using three different restriction enzymes. DNA was extracted from 45 blood samples of Sudanese camels (15 form each breed). Polymerase chain reaction was done using specific primers in order to amplify the D-Loop region. The PCR products were digested using 3 different restriction enzymes (tag1, hinf and scal). The products were run on agarose gel 2%. The result of this study revealed differences between the three breeds according to digested and undigested samples. Tag1 did not cut any of the 45 samples of all breeds, while scal cut 4.4% of the Shanabla breed, 6.7% of Kababeish breed, but did not cut any of Nyalawei camels. The third restriction enzyme hinf cut all sample from Shanabla camels, and also cut 95.6% of Kababeish breed and also 95.6% of Nyalawei camels.

This study was conducted on 45 heads (15 of each breed) of three Sudanese domestic goat breeds (Desert goat, Nubian goat and Taggar goat) to pursue complete mitochondrial DNA analysis using PCR-RFLP method to detect the mitochondrial DNA diversity of two mit-DNA haplogroups, lineage A and lineage D. The results revealed that most individuals of the three breeds belonged to lineage A with frequencies 0.2 of Desert goats, 0.6 of Nubian goats and 0.6 of Tagar goats. Some of the individuals belonged to modified lineage A with frequencies 0.2 of Desert goats, 0.2 of Nubian goats and 0.2 of Tagar goats; no individual of each belonged to lineage D. In conclusion, the appearance of individuals that belonged to modified lineage A in the different goat breeds might be due to mutation resulted from insertion or deletion of events and might a criterion of the Sudanese indigenous goat breeds. However this suggestion needs more confirmative analysis.

No abstract