Pseudomonas aeruginosa is one of the most common gram-negative microorganisms identified in the clinical specimens of hospital admitted patients. This was a retrospective study done among the patients attending Dhaka Medical College Hospital during January to December, 2006. A total 294 strains of P. aeruginosa were isolated of which 206 (70%) were from admitted patients and 88 (30%) from outpatients. Some 175 (60%) of the isolates were from males while 119 (40%) from females. Majority of the patients (141, 41.2%) were aged between 21-40 years and 102 (34.7%) were below 20 years. A significant proportion (about 45%) of the patients were from casualty wards, surgical outdoor, ear-nose-throat outdoor and burn unit. Four of the clinical specimens including wound swab, pus, aural swab and urine comprised of 279 (95%) of the total samples. The bacteria isolated were identified by colony morphology, microscopy and relevant biochemical tests. Antimicrobial sensitivity pattern was tested using standard guidelines. Almost all of the P. aeruginosa isolates were resistant to cefixime (93.3%) and co-trimoxazole (93.5%), majority were resistant to ceftazidime (86.8%), gentamycin (77.3%) and ciprofloxacin (75.5%). The result of the study shows that imipenem is the most effective drugagainst P. aeruginosa, followed by amikacin and ciprofloxacin.Bangladesh J Med Microbiol 2007; 01 (02): 48-51

H. pylori infection occurs worldwide. Approximately 50% of the world population is infected with this organism. A cross-sectional study was conducted on 81 dyspeptic patients attending at Dhaka Medical College Hospital, for diagnostic endoscopy. H. pylori infection was measured by three invasive methods: culture, rapid urease test & histopathology. Among study patients maximum (34.57%) H. pylori infected were in 21-30 years age group, bearing male female ratio 1: 92.86. Forty four (54.32%) out of 81 were culture positive, 61 (75.31%) were rapid urease test positive and 62 (76.54%) were histopathology positive. By using 'gold standard' definition, 64 (79.02%) were H. pylori infected, 17 were un-infected. Finally comparing among three invasive methods, all are highly sensitive and specific to diagnose H. pylori infection.

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This study was undertaken to find media better than routinely used media in isolation of uropathogens.Three hundred urine samples having pus cells > _ 5/ HPF were enrolled for the study. Comparison of isolation and identification of uropathogens among HiCrome UTI Agar media, 5% Sheep Blood agar & MacConkey agar and CLED agar media were done. Among them 95(31.67%) samples showed single growth, 6 (2%) showed mixed growth and 199 (66.67%) showed no growth. Rate of presumptive identification of organisms in primary culture plate were high in HiCrome UTI agar media. For Escherichia coli, it was 94.20% whereas in CLED agar it was 79.71% and by Blood agar and MacConkey agar media in combination it was 82.61%. All the Enterococcus spp. were identified in HiCrome UTI agar media, 33.33% in CLED agar media but none in Blood agar and MacConkey agar media. Among the mixed growth, 100% organisms were identified on HiCrome UTI Agar media due to distinct colour produced by the different organisms, whereas in one (16.67%) sample (mixed Esch.coli and Pseudomonas spp.) organisms were identified on other three media.

The present study was undertaken to establish the efficacy of acridine orange staining for diagnosis of Bacterial Vaginosis (BV) and association of Gardnerella Vaginalis with BV. Two hundred sexually active females in the age group of 15-45 years, with vaginal discharge and itching, were selected for the study.