Most of the formulations in the Siddha system is very effective but they have not been standardized. Any drug should be standardized before clinical use. There are various formulations in Siddha to treat cardiovascular diseases. One among them is the poly-herbal formulation Maaradiappuku Chooranam, but it has not been validated so far. The principal intention of this current study is to regularize the drug ‘Maaradaippuku chooranam’ by physicochemical, phytochemical and biochemical evaluation through modern Analytical techniques. The organoleptic characteristics of the Chooranam are in solid form which is pale brownish in colour and soft to touch with a characteristic odour which reveals the quality of the formulation. The aflatoxin assay showed the chooranam is free of aflatoxins. The outcomes of the study for pesticide residue show no traces of pesticide residue in the chooranam. This polyherbal formulation does not contain microbial contamination and displays the existence of steroids, alkaloids, triterpenoids, phenols, tannin, saponin and sugar. The ash value is 9.5 ± 0.4%. Heavy metals like Arsenic, Lead, Mercury and Cadmium are below the detectable limit.
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