Most of the formulations in the Siddha system is very effective but they have not been standardized. Any drug should be standardized before clinical use. There are various formulations in Siddha to treat cardiovascular diseases. One among them is the poly-herbal formulation Maaradiappuku Chooranam, but it has not been validated so far. The principal intention of this current study is to regularize the drug ‘Maaradaippuku chooranam’ by physicochemical, phytochemical and biochemical evaluation through modern Analytical techniques. The organoleptic characteristics of the Chooranam are in solid form which is pale brownish in colour and soft to touch with a characteristic odour which reveals the quality of the formulation. The aflatoxin assay showed the chooranam is free of aflatoxins. The outcomes of the study for pesticide residue show no traces of pesticide residue in the chooranam. This polyherbal formulation does not contain microbial contamination and displays the existence of steroids, alkaloids, triterpenoids, phenols, tannin, saponin and sugar. The ash value is 9.5 ± 0.4%. Heavy metals like Arsenic, Lead, Mercury and Cadmium are below the detectable limit.

Siddha drugs are natural products obtained from herbal, herbo-mineral, mineral and animal kingdom. Most of the Siddha medicines are effective but they have not been standardized yet. So, there be in need to progress a standardization technique. Raja Elathi Chooranam is a poly-herbal formulation mentioned in the classical Siddha literature for the medication of Menorrhagia. The aim of this study to estimate quality of Raja Elathi Chooranam by conducting physicochemical analysis, phytochemical analysis and other analytical techniques. This study was done based on Pharmacopoeial laboratory for Indian Medicine guidelines. High performance thin layer chromatography was done through CAMAG software. The organoleptic character of the sample drug justifies the solid and its aromatic and fine powder nature confirms that the formulation is of good quality. The results obtained after phytochemical analysis of test drug showed 2.427 ± 0.4839 % of Loss on drying at 105°C, 9.633 ± 0.7506% of Total Ash, 0.054 ± 0.01652 % of Acid insoluble ash etc. The pH value of the drug 6.5 which is indicates it is weakly acidic. The results obtained after preliminary phytochemical analysis confirmed the presence of alkaloids, flavonoids, steroids, triterpenoids, coumarin, phenols, tannins, saponins and sugar. Biochemical analysis of the test drug reveals the presence of Carbonates, Chlorides, Sulphates and Phosphates. The test drug is devoid of microbial contamination, heavy metals and pesticide residues were below quantification limit. From the result we infer that the drug is of standard quality and shall be used as reference in pharmacopoeia standards. Key words: Standardization, Raja Elathi Chooranam, Poly herbal, Siddha, Physicochemical analysis, phytochemical analysis.