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Biomimetic nanoparticles emerge as an auspicious class of antioxidative nano‐agents owing to their inherent catalytic properties. Among them, nanoceria revolutionizes the field of nanomedicine due to its astonishing antioxidant enzyme mimetic activities. However, the complete pharmacological potential of nanoceria needs to be explored yet. In this work, antioxidant and cytoprotective potentials of nanoceria against H2O2 induced oxidative stress was examined both in fibroblast cells and in zebrafish. Furthermore, the cellular uptake, cell cycle, mitochondrial membrane potential, morphological and nuclear analysis suggests that the nanoceria successfully defend the cells against oxidative stress induced cell death. Thus, the present study opens up new avenue of nanoceria for future biomedical applications.

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This paper reports on the real‐time imaging and developmental biochemistry of the freshwater caridean shrimp, Caridina pseudogracilirostris. The complete time‐lapse development of a single embryo was recorded in an artificial mold, developed in our lab, and imaged under a stereomicroscope. It took 8 days to complete the 5 stages of embryonic development (1 cleavage stage, 2 gastrulation stage, 3 nauplius stage, 4 prehatching embryo, and 5 zoea stages). As the decapod eggs are enriched with dense yolk, biochemical determination of the major components was made to evaluate the yolk utilization during embryogenesis. The concentration of protein, lipid, and carbohydrate declined drastically from Stage I (cleavage) to Stage IV (Zoea), reflecting sustained yolk utilization during embryogenesis. The increase in the size of the embryo correlates with changes in water content. Lipids, being the principal organic substrate, changes in the fatty acid (FA) composition of embryos during development were determined by GC. The FA composition was observed within the range of 25%–60.87% for saturated, 22.57%–56.45% for monounsaturated, and 5.64%–18.51% for total polyunsaturated FAs. The essential polyunsaturated fatty acid were higher in Stages I, IV, and V, suggesting a major role in embryogenesis. The cellular proliferation and organogenesis as visualized in the real‐time imaging correlate well with the biochemical variations observed in C. pseudogracilirostris.

The caridean shrimp Caridina pseudogracilirostris (order-Decapoda, family-Atyidae) is commonly found in the brackish waters of the southwestern coastal regions of peninsular India. The draft genome sequencing of this shrimp was carried out by Illumina NovaSeq6000 platform. We obtained a draft genome assembly of C. pseudogracilirostris (1.3 Gbp; 6,03,962 scaffolds; scaffold N50 = 2641 bp; 35.71% GC, 52.8% BUSCO completeness). It revealed that 24.60% of the genomic sequences are repetitive and has high proportion of simple sequence repeats (SSR) spanning 7.26% of the entire genome. Other major repeat classes found are Retroelements (3.19%), LINEs (2.37%) and L2/CR1/Rex (1.05%). A total of 14101 genes were identified with AUGUSTUS. The predicted genes were functionally annotated using EggNOG-mapper and the genes containing database hits were sorted based on the biological processes using Panther database. Genes associated with developmental process (31), cellular process (30), immune system process, (20) and reproductive process (24) were further analyzed in pathway commons and narrowed down into genes involved in regulatory pathways. We have conducted a comparative study with 15 crustacean species using OrthoFinder. It provided the phylogenetic species tree and a total of 7396 orthogroups were identified. C. pseudogracilirostris has shown only 3.7% orthologous genes.