The effect of sodium bicarbonate on CBF and intracellular pH in man: Stable Xe-CT and 31P-MRS. Acta Neurol Scand 1996: Suppl. 166: 96-98The effects of sodium bicarbonate on cerebral blood flow (CBF) and intracellular pH were studied in five normal volunteers. CBF and intracellular pH were measured by stable xenon computed tomography and phosphorus-3 1 magnetic resonance spectroscopy (31P-MRS) respectively. Each patient received 7% sodium bicarbonate (3.5 mVkg body weight) infused intravenously for 15 minutes. Before and after this injection, CBF, intracellular pH and physiological parameters were measured. CBF and PaC02 were significantly increased. On the other hand, hematocrit and intracellular pH were decreased. These result suggests that three factors are thought to contribute to increase CBF during administration of sodium bicarbonate in humans: 1) arterial dilatation in response to carbon dioxide 2) an decrease of hematocrit 3 ) intracellular acidosis.

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It is widely believed that evolutionarily advanced cells require nuclei rich in DNA to proliferate, while mitochondria isolated from nuclei cannot proliferate. We have developed a novel stable cell line, designated "mitochondrial cells (MitoCells)", maintaining active mitochondria, the majority of which lacked nuclei. MitoCells can be continuously generated in culture. Herein, we report proliferation studies of MitoCells. We found that the nuclear DNA-less (nDNA-less) MitoCells, which were sorted with flow cytometry and were filtrated with 1.2 μmeter and 3 μmeter isopore membrane filters, could exist and proliferate. We also confirmed that the sorted nDNA-less MitoCells had mitochondrial DNA(mtDNA) with T9176C mutation analysis and that the unsorted MitoCells had SURF-1 gene of nDNA. These results suggest that MitoCells could have prokaryotic nature with both mtDNA and nDNA.

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