The numbers of T, B, and ‘null’ blood lymphocytes were determined in 216 normal healthy persons, whose ages ranged from 20 to 94 years. No change was found in the numbers of T or B lymphocytes with increasing age of the subjects, whereas both the total lymphocytes and the number of ‘null’ lymphocytes decreased just significantly with age (p < 0.05). The response of blood lymphocytes to phytohaemagglutinin in vitro was measured in 25 subjects aged 20–30 years and compared with the response in 23 subjects aged over 60 years. There was no significant difference in the response of the two groups.

Introduction Triglyceride (TG) is an independent risk factor for coronary heart disease. Previous work has shown that short-term supplementations of mouse chow with oxidized linoleic acid (OxLA) significantly reduce the level of plasma triglycerides. Study Objective This study aims to determine the effects of longer-term supplementation of mouse chow with various concentrations of oxidized linoleic acid (OxLA) on plasma triglycerides. Study Design The study consisted of forty C57BL/6 wildtype mice divided into four groups (n = 10). Two groups were kept as controls. One control group (P) was fed plain chow and the second control group (C) was fed chow supplemented with linoleic acid. The other two experimental groups (A) and (B) were fed oxidized linoleic acid supplemented chow in the following doses: 9 mg/day of oxidized linoleic acid and 18 mg/day of oxidized linoleic acid/mouse. Results and Conclusion Mice that were on a diet supplemented with the higher dose of oxidized linoleic acid showed a 39% decrease in hepatic PPAR-α and a significant decrease in the plasma HDL levels compared to the mice that were fed diets of plain and linoleic acid supplemented chow. Interestingly, the longer-term consumption of oxidized linoleic acid may predispose to atheropathogenesis.

BackgroundWe have previously shown that oxidized linoleic acid significantly lower plasma triglyceride (TG) levels as compared to animals fed oleic acid, the changes was associated with increased APOA5 and acetyl‐CoA oxidase genes expression in the mice. The focus of the current study is to determine the mechanisms and plasma and tissue metabolites associated with the plasma triglycerides decreased levels.Study DesignWe fed C57BL6 normal male mice 4 groups (N=10 each) 4 different types of mouse diet formulations as follows: normal mouse chow (P), normal chow supplemented with 18 mg/day linoleic acid (C), oxidized linoleic acid 9 mg (A) or 18 mg /animal/day) for 2 months. Mice were sacrificed; blood and tissues (liver, adipose, kidney, and heart) were collected. We measured the plasma lipids, performed Western Blot for the Apo5, ApoC3 and related proteins expression.Results and ConclusionThis is a going study, we currently completed the plasma APoC3 measurements; as anticipated our data have shown a significant dose dependent decreases in plasma ApC3 on mice fed low (P<0.01) and high (P<0.003) dose oxidized linoleic acid compared to the control group on linoleic acid. These finding indicate for the first time that intake of oxidized linoleic acid decreases plasma ApoC3 levels and modulatesplasma TG..