Aim: The objective of this study was to assess the Anti-ulcer Activity of the Polyherbal Formulation made from the seeds of Cumin Cyminum, Trigonella Foenum Graecum, Piper Nigrum L. and rhizomes of Curcuma Longa in Wistar albino rats. Methods: Male Albino Rat of Wistar strain were used to evaluate the Polyherbal Formulation’s anti-ulcer properties utilising an ethanol-induced gastric ulcer model. The effectiveness of PHF was assessed by determining, gastric pH and gastric mucus, GSH, malondialdehyde (MDA), and nitric oxide (NO) levels in stomach tissues. Results: The administration of the 400 mg/kg dosage of polyherbal formulation significantly exhibits the gastroprotective effects, when compared to the ethanol group. Conclusion: PHF, containing seeds extracts of Cumin Cyminum, Trigonella Foenum Graecum, Piper Nigrum L. and rhizomes of Curcuma Longa was found to possess antiulcer properties in ethanol induced experimental animal models of gastric ulcers, and these findings suggest that the significant gastroprotective activity could be mediated by its antioxidant activity.