Hospital environments contain different types of microorganisms. Airborne fungal are one of these microbes and the major sources of hospitals indoor contamination that will be able to cause airborne fungal diseases. The total count and diversity of the airborne filamentous and yeasts fungi were investigated in indoor and outdoor air of selective wards of Emam Reza Educational, Research and Treatment Center. This cross-sectional study was performed during the fall season. One hundred and ninety-two environmental samples of indoor and outdoor air from hematology, infectious diseases, Ear, Nose and Throat (ENT) and Neonatal Intensive Care Unit (NICU) wards were collected by open plate technique (on Sabouraud dextrose agar media) once a week. The cultures were then examined and evaluated according to macroscopic and microscopic examination criteria. In this study, 67 (62.03%) of indoor samples and 81 (96.42%) of outdoor samples were positive for fungi. The most isolated fungi were yeast species (17.12%), <em>Penicillium</em> spp. (16.34%), <em>Alternaria</em> spp. (14.39%), <em>A.</em> <em>niger</em> (11.28%), <em>A</em>. <em>flavus</em> (8.95%), respectively. Almost all of the wards showed high rates of contamination by various fungi. However, the analysis of the data showed that indoor air of hematology ward had the highest fungal pollution. In contrast, the outdoor air of ENT had the highest fungal pollution. Thus, these results demonstrated that the cleansing and disinfection procedures in the hospital wards should be improved yet.

Hospital ward environments contain various types of microorganisms, in which fungal agents are one of the main contaminants that may cause hospital-acquired infections. Regarding this, the aim of the present study was to evaluate the effect of nanosilver paint on reducing fungal contaminants of indoor air in an educational, research, and treatment center. Two rooms in the hematology ward were selected. One room was painted using usual paint (control room) and the other room was painted with paint containing nanosilver particles (experimental room). One hundred and twelve samples were collected using active (Anderson BioSampler) and passive (settle plate or open plate) air sampling techniques. The samples were incubated for 3–7 days at 35°C, and the positive fungal cultures were examined according to morphological and microscopic characteristics. Following active sampling, the mean and standard deviation of the number of colony-forming units (CFU/m3) of fungi colonies in the experimental and control rooms were 29.21 ± 17.99 and 22.50 ± 10.02 before intervention and 13.79 ± 6.20 and 31.07 ± 21.1 after intervention, respectively. Following passive sampling, the number of CFU/plate in the experimental and control rooms was 6 and 0 before and 1and 1 after intervention, respectively. The use of the nanosilver paint was effective in reducing air fungal contamination. Moreover, the active sampling method was more sensitive to measuring the concentration changes for fungal bioaerosols.