In order to ensure the timely and uninterrupted supply of medicinal plant raw materials, the methods of cultivation of plant cell cultures, namely, the production of plant root cultures, are relevant. In this paper, the geroprotective potential of Hedysarum neglectum Ledeb and Panax ginseng C. A. Mey root cultures is studied. They were cultured under in vitro conditions by transforming the rhizome (H. neglectum) and seed seedlings (P. ginseng) with Agrobacterium rhizogenes 15834 Swiss. To identify the geroprotective potential, the antimicrobial disc-diffusion method and the antioxidant activity were analyzed by titration of KMnO4 extracts of plant root cultures. The qualitative and quantitative composition was analyzed using high-performance liquid chromatography, thin-layer chromatography, and gas chromatography with mass spectrometry. In the course of the work, the presence of antimicrobial and antioxidant activity of plant root culture extracts was established. Biologically active substances contained in extracts of Hedysarum neglectum Ledeb root crops and Panax ginseng C. A. Mey are characterized by geroprotective potential, so they can act as a source of natural antioxidants in the functional nutrition of the geroprotective orientation.

Introduction. There is an urgent need for geroprotectors that prevent premature aging, especially antioxidants of plant origin. Due to the shortage of medicinal plant materials, scientists look for alternative sources of bioactive compounds of phenolic nature, for example, cell cultures and organs of higher plants. This paper describes a study of the geroprotective potential of in vitro bioactive compounds isolated from yarrow (Achilleae millefolii L.) cell cultures. Study objects and methods. Callus, suspension and hairy root cultures of A. millefolii were obtained by in vitro cultivation on modified nutrient media. High performance liquid chromatography (HPLC) was used to analyze the composition of the cell cultures and ethanol extracts. The extracts’ antimicrobial activity was studied by the disk diffusion method and their antioxidant activity was measured based on titration of a potassium permanganate solution. Results and discussion. The biomass of all yarrow cell cultures contained essential oils, flavonoids, glycosides, phenolic acids, carotenoids, as well as vitamins C and E. The suspension culture had a higher content of essential oils, flavonoids and glycosides than the callus and hairy root cultures. The extracts of the A. millefolii suspension culture also contained geroprotectors – phenylpropanoids, flavonoids, and simple phenols, with a prevalence of caffeic acid, cynaroside, 4,5-dicofeylquinic acid, apigenin, and luteolin. In addition, HPLC revealed the presence of cumic aldehyde, umbelliferone, 3-caffeylquinic acid, and caffeic acid – the bioactive compounds previously not reported in yarrow. In vitro experiments with the extracts proved their antimicrobial and antioxidant activity. Conclusion. The complex of bioactive compounds isolated from the biomass of yarrow suspension culture provides this plant with potential geroprotective properties. Thus, yarrow can be used to create nutraceuticals that prevent premature aging.

Purpose: To describe the advantages and disadvantages of mountain ash as a component of functional nutrition.

Objective: to identify the effect of various concentrations fragrant herbs extracts additives of on the content of polyphenols and antioxidant activity of the tea mushroom-based drink (kombucha)

Global technological progress is one of the main problems of ecosystem deterioration. The high concentration of industrial enterprises contributes to the development of the region's environmental crisis and the decline in public health. An increase in the environmental pollutant contributes to the activation of oxidative stress in the human body, which plays a key role in the pathogenesis of a number of chronic diseases (cancer, diabetes, cardiovascular diseases, etc.), leading to a decrease in life expectancy and early aging of the body. To increase the adaptive abilities of the human body, it is important to use plant raw materials. A promising source of natural nutrients is the Hedysarum neglectum Ledeb. In order to obtain the extract of the forgotten kopek, the method of extraction was used at room temperature with a reverse refrigerator for 2 hours. In this study, to determine the working parameters of extraction (type of extractant, concentration of extractant), a selection of various extractive substances was selected. The efficiency of the extraction process was determined by two parameters: extractivity and yield of biologically active substances. The maximum extractivity was observed when using 50 % ethyl alcohol.To determine the working concentration of the water-ethanol mixture, the prepared extracts were analyzed for the content of flavonoids, tannins, and ascorbic acid. The maximum yield of biologically active substances (flavonoids 419.02±3.58 mg%, tannins 8.10±1.03 %, ascorbic acid 691.04±3.62 mg/kg) was observed during extraction with 50% water-ethanol mixture. The obtained results suggest that the water-ethanol extract of Hedysarum neglectum Ledeb. it is a potential source of natural biologically active substances for creating functional food products.