No abstract

Although geopropolis has been the subject of many chemical and pharmacological studies, there are few studies investigating the photoprotective activity of formulations containing propolis. Thus, we investigated in vivo the photoprotective efficacy of the cream containing geopropolis extract by macroscopic and histological evaluation of the skin of Wistar rats subjected to ultraviolet radiation (UVB). We also evaluated the chemical composition of hydroethanolic extract using the HPLC-DADESI-MS/MS technique, as well as antioxidant activity by the photocolorimetric method of free radical DPPH (2.2-diphenyl-1-picrylhydrazine) and cytotoxic activity by the in vitro MTT quantitative method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The extract had a varied chemical composition, 29 different phenolic compounds being detected, distributed between phenols and flavonoids, the latter being represented by chalcones, flavones and flavonols. The highest percentages of DPPH inhibition e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. The hydroethanolic extract did not exert cytotoxic effects since high percentages of viability of L929 fibroblasts were observed after incubation for 72 hours at different concentrations of the extract.  On skin submitted to cream application containing of geopropolis extract and the irradiation with UVB did not occur macroscopic and histological lesions. Thus, we concluded that the cream containing of geopropolis extract produced by Melipona subnitida was able to protect the skin from lesions induced by UVB irradiation, thus demonstrating photoprotective effect.

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Research on the chemical composition and pharmacological activities of geopropolis produced by stingless bees (Hymenoptera, Apidae, Meliponini) may contribute to expand its use of propolis-based formulations in the clinical context. Thus he study aimed to evaluate the chemical composition and biological activity of the hydroethanolic extract (HEG) of the geopropolis of Partamona cupira, obtained in the semiarid region of northeast Brazil. Chemical analyses of HEG were carried out using HPLC-DADESI-MS/MS. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxic activity by the in vitro MTT method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The antibacterial activity of the HEG was evaluated through the disc-diffusion test on agar and measurement of the promoted by the extract in different concentrations. The genoprotective potential of the HEG was evaluated through the comet assay on fibroblasts of L929, co-treated with the extract and submitted to genotoxicity induction with H2O2. We also investigated the healing effect of the cream containing geopropolis (10%) on experimental skin wounds in Wistar rats. The HEG presented in its composition phenolic compounds of high biological activity, as well as revealed high antioxidant activity and promoted genoprotective effect by reducing DNA damage from L929 fibroblasts. The HEG presented antimicrobial activity promoting inhibition of S. aureus, S. pyogenes, E. coli and E. aerogenes. The topical use of the cream containing geopropolis promoted wound closure and faster reepithelialization in relation to the control group, in addition to a less intense inflammatory reaction, increased fibroblastic proliferation and collagen deposition.

The objective of this study was to describe the clinical and pathological aspects of diseases of the digestive system in agoutis (Dasyprocta leporina Linnaeus, 1758) diagnosed by the “Laboratório de Patologia Veterinária” (Veterinary Pathology Laboratory) of the “Universidade Federal Rural do Semi-Árido” (UFERSA), from January 2018 to February 2020. During the study period, necropsy and a survey of the clinical history of 27 agoutis were performed, 25.93% (7/27) of which were diagnosed with digestive system diseases. The percentages of digestive tract diseases among the diagnosed were: acute carbohydrate overload (11.12%), gastric ulcer (7.41%), gastric volvulus (3.70%), and intestinal volvulus (3.70%). Studies on the occurrence rate of these diseases, as well as the description of their clinical and anatomopathological aspects, may serve as a basis for guiding the appropriate management in the breeding of these animals.