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Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.

Overexpression of the platelet-derived growth factor receptor (PDGFR) was already associated with the loss of p53 function as cancer progresses in lung, breast, and cervical cancers. Cancer biomarker detection has faced challenges and barriers due to various limitations, including a high limit of detection, low sensitivity, time-consuming techniques, and expensive equipment. Hence, the present investigation is designed to develop a cost-effective novel biosensor based on a charge-based affinity bait molecule to detect the PDGFR, thus overcoming the limitations and challenges with an immune technique based on antigen–antibody interactions. We employed EDC–NHS coupling between poly (diallyl dimethylammonium chloride) and poly­(acrylic acid) to attach the multiwall carbon nanotube surface. As a result, we performed electrochemical PDGFR conversion sensing with a dynamic range of 1–10,000 ng/mL and a detection limit of 1.5 pg/mL, which is comparable to the best current results. The biosensor also displayed good selectivity, 2.51% repeatability (RSD, n = 5), and 30 days of stability. Our study provides a pathway for the design of diagnostic interfaces in biosystems, as well as the emergence of new sensor types based on ligand–receptor interactions.

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Methyl orange dye-doped K2SO4 single crystal has been synthesized by a slow evaporation method, and its properties have been investigated. The powder X-ray diffraction (PXRD) studies on the single crystal confirmed the crystalline property, noncentrosymmetric system, and the space group P63/mmc (D64th) of crystal. The reaction involved in the functional group of the grown crystal has been confirmed from the FTIR analysis. The optical properties of absorbance and band gap were calculated from the UV-Vis analysis. The obtained materials were identified from the EDX analysis. The electron transformation and optical distortion were identified at 270 nm in the photoluminescence study. The transmission electron microscopy method analyzed the morphology of the grown crystal. The dielectrics of the grown crystal were studied. The effect of temperature on the grown crystal was studied using the TG/DTA analysis. The bacterial susceptibility of the grown crystal was evaluated from Gram-positive and Gram-negative bacteria. All the results demonstrated that the grown crystal is suitable for optical, electronic, and bacterial applications.