No abstract

Understanding and mitigating drivers' negative emotions, stress levels, and anxiety is of high importance for decreasing accident rates, enhancing road safety, and providing a healthy lifestyle to the community of drivers. While detecting drivers' stress and negative emotions can significantly help with this goal, understanding what might be associated with increases in drivers' negative emotions and high stress level, might better help with planning interventions. While studies have provided significant insight into detecting drivers' emotions and stress levels; not many studies focused on the reasons behind changes in stress levels and negative emotions. In this study, by using a naturalistic driving study database, we analyze the changes in the driving scene, including road objects and the dynamical relationship between the ego vehicle and the lead vehicle with respect to changes in drivers' psychophysiological metrics (i.e., heart rate (HR) and facial expressions). We find that different road objects might be associated with varying levels of increase in drivers' HR as well as different proportions of negative facial emotions detected through computer vision. Our results indicate that larger vehicles on the road, such as trucks and buses, are associated with the highest amount of increase in drivers' HR as well as negative emotions. Additionally, we provide evidence that shorter distances to the lead vehicle in naturalistic driving, as well as the higher standard deviation in the distance, might be associated with a higher number of abrupt increases in drivers' HR, depicting a possible increase in stress level. Lastly, our results indicate more positive emotions, lower facial engagement, and a lower abrupt increase in HR at a higher speed of driving, which often happens in highway driving. Our results collectively show that driving at higher speeds happening in highways by avoiding certain road objects might be a better fit for keeping drivers in a calmer, more positive state. This research lays the ground for designing human-centered vehicles, urban environments, and services that can understand the level to which each road object and environment might affect drivers' and passengers' wellbeing.

No abstract

The function of dopaminergic neurons in the substantia nigra is of central importance to the coordination of movement by the brain's basal ganglia circuitry. This is evidenced by the loss of these neurons, resulting in the cardinal motor deficits associated with Parkinson's disease. In order to fully understand the physiology of these key neurons and develop potential therapies for their loss, it is essential to determine if and how dopaminergic neurons are replenished in the adult brain. Recent work has presented evidence for adult neurogenesis of these neurons by Nestin+/Sox2– neural progenitor cells. We sought to further validate this finding and explore a potential atypical origin for these progenitor cells. Since neural progenitor cells have a proximal association with the vasculature of the brain and subsets of endothelial cells are Nestin+, we hypothesized that dopaminergic neural progenitors might share a common cell lineage. Therefore, we employed a VE-cadherin promoter-driven CREERT2:THlox/THlox transgenic mouse line to ablate the tyrosine hydroxylase gene from endothelial cells in adult animals. After 26 weeks, but not 13 weeks, following the genetic blockade of tyrosine hydroxylase expression in VE-cadherin+ cells, we observed a significant reduction in tyrosine hydroxylase+ neurons in the substantia nigra. The results from this genetic lineage tracing study suggest that dopaminergic neurons are replenished in adult mice by a VE-cadherin+ progenitor cell population potentially arising from an endothelial lineage.