Human‐induced pluripotent stem cells‐derived hepatocyte‐like cells (hiPSCs‐HLCs) holds considerable promise for future clinical personalized therapy of liver disease. However, the low engraftment of these cells in the damaged liver microenvironment is still an obstacle for potential application. In this study, we explored the effectiveness of decellularized amniotic membrane (dAM) matrices for culturing of iPSCs and promoting their differentiation into HLCs. The DNA content assay and histological evaluation indicated that cellular and nuclear residues were efficiently eliminated and the AM extracellular matrix component was maintained during decelluarization. DAM matrices were developed as three‐dimensional scaffolds and hiPSCs were seeded into these scaffolds in defined induction media. In dAM scaffolds, hiPSCs‐HLCs gradually took a typical shape of hepatocytes (polygonal morphology). HiPSCs‐HLCs that were cultured into dAM scaffolds showed a higher level of hepatic markers than those cultured in tissue culture plates (TCPs). Moreover, functional activities in term of albumin and urea synthesis and CYP3A activity were significantly higher in dAM scaffolds than TCPs over the same differentiation period. Thus, based on our results, dAM scaffold might have a considerable potential in liver tissue engineering, because it can improve hepatic differentiation of hiPSCs which exhibited higher level of the hepatic marker and more stable metabolic functions.
Bioactive scaffolds that can increase transplanted cell survival time at the defect site have a great promising potential to use clinically since tissue regeneration or secretions crucially depend on the transplanted cell survival. In this study embedded basic fibroblast growth factor (bFGF)‐polycaprolactone‐polyvinylidene fluoride (PCL‐PVDF) hybrid was designed and fabricated by electrospinning as a bio‐functional nanofibrous scaffold for bone tissue engineering. After morphological characterization of the PCL‐PVDF (bFGF) scaffold, nanofibers biocompatibility was investigated by culturing of the human induced pluripotent stem cells (iPSCs). Then, the bone differentiation capacity of the iPSCs was evaluated when grown on the PCL‐PVDF and PCL‐PVDF (bFGF) scaffolds in comparison with culture plate as a control using evaluating of the common osteogenic markers. The viability assay displayed a significant increase in iPSCs survival rate when grown on the bFGF content scaffold. The highest alkaline phosphatase activity and mineralization were detected in the iPSCs while grown on the PCL‐PVDF (bFGF) scaffolds. Obtained results from gene and protein expression were also demonstrated the higher osteoinductive property of the bFGF content scaffold compared with the scaffold without it. According to the results, the release of bFGF from PCL‐PVDF nanofibers increased survival and proliferation rate of the iPSCs, which followed by an increase in its osteogenic differentiation potential. Taking together, PCL‐PVDF (bFGF) nanofibrous scaffold demonstrated that can be noted as a promising candidate for treating the bone lesions by tissue engineering products.
:
P-Coumaric acid (p-CA) is a hydroxycinnamic acid, an organic compound that is a hydroxyl derivative of
cinnamic acid. P-CA is the most abundant isomer of the three in nature and can be found in a wide variety of edible plants
such as fungi, peanuts, navy beans, tomatoes, carrots, basil, and garlic. Recently, the therapeutic properties of p-CA have
received a great deal of attention from the scientific society. Here, we described the medicinal effects of p-CA on various
pathological conditions. This review was performed via evaluating PubMed reported studies from January 2010 to January
2020 also reference lists were checked to find additional studies. All intermediation or complementarity of animal models,
case-control and cohort studies, in-vitro studies, and controlled trials (CTs) on p-CA were acceptable, although, plant extract
studies without indication of main active substances were excluded due to the considerable diversities and heterogeneities.
According to recent evidence regarding the beneficial effects of p-CA, numerous diseases such as nephropathies,
cardiovascular diseases, neuro-inflammatory diseases, liver diseases, cancers, and some metabolic disorders could
potentially control by this natural herb. Interestingly, autophagy is a novel molecular mechanism involved in the crosstalk
between classic effects of p-CA and introduces alternative therapeutic pathways for this compound. Much work remains in clarifying the main therapeutic properties among the various p-CA effects; these will be the subject of forthcoming work,
which could be resulting in presenting the further mechanism of action.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.