ResumoA bioquímica sérica permite estabelecer valores de referência utilizados na avaliação clínica e metabólica de bezerros, reduzindo as perdas na criação bovina. Neste contexto, objetivou-se avaliar o perfil bioquímico sérico e a influência do fator etário em bezerros da raça Senepol, do nascimento até 120 dias de idade. O experimento foi realizado no município de Uberlândia-MG, Brasil, em quatro momentos após o nascimento (0 -15º, 30º, 60º e 120º dia de vida). As amostras de sangue foram obtidas por venopunção da jugular para determinação das concentrações séricas de proteínas totais, albumina, globulina, triglicerídeos, colesterol, AST, GGT, FA, cálcio, fósforo, magnésio, creatinina e uréia. O colesterol manteve-se abaixo ou nos valores mínimos de referência em todos os momentos. Os animais aos 30 dias apresentaram os valores mais baixos do proteinograma, minerais e triglicerídeos que se normalizaram aos 60 dias de vida, exceto a albumina que apresentou recuperação mais lenta. Concluiuse que há influência da faixa etária no comportamento dos constituintes bioquímicos analisados e que há diferenças quando comparados a outras raças. Palavras-chave: Bezerros, bioquímica sérica, colesterol, Bos taurus abstract The serum biochemical values allow to establish reference used in metabolic and clinical evaluations of calves, reducing losses in cattle breeding. The aim of this study was to evaluate the influence of age on serum biochemical profile in Senepol calves, at the first 120 days of age. The experiment was conducted in the city of Uberlândia, Minas Gerais, Brazil, in four periods after birth (0-15, 30, 60 and 120 days of age). Blood samples were obtained by jugular venipuncture for determination of serum total protein, albumin, globulin, triglycerides, cholesterol, AST, GGT, FA, calcium, phosphorus, magnesium, creatinine and urea. Cholesterol remained at minimum reference values or below throughout the experiment. Animals at 30 days showed the lowest values of protein concentrations, minerals and triglycerides that normalized up to 60 days of life, except to albumin that slower recovery was showed. Our data showed that the serum biochemical profile in Senepol is influenced by age up to 120 days of age; moreover there are differences with other breeds.

This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species of equid was the only factor that contributed to an elevated seroprevalence of Leishmania spp.

Neospora caninum causes reproductive disorders in dairy cattle such as repeat breeding, abortion, and anestrus, which are risk factors associated with the seroprevalence of N. caninum in Brazil and worldwide. This study aimed to verify the risk factors related to the seroprevalence of Neospora caninum in crossbred dairy cows in the city of Uberlândia, Minas Gerais. During, January to July 2013, 740 blood samples were collected from 57 herds with a history of reproductive disorders and determinations of antibody titers against N. caninum were obtained by ELISA assay. The seroprevalence of N. caninum was 18.4% (136/740). Risk factors for N. caninum were cows greater than 2 years of age (P = 0.034), mechanical milking (P = 0.012), and frequent purchase of animals (P = 0.004), in addition to individual history of abortion (P = 0.007) and repeat breeding (P = 0.030). The highest probability of occurrence of associated risk factors was associated with individual history of abortion and repeated breeding and the frequent purchase of animals, with odds of 12.8, 5.9, and 3.8 times, respectively. In conclusion, the risk factors associated with N. caninum: members of the female sex and cows with more than 2 yearsof-age, mechanical milking, and the frequent purchase of animals, in addition to individual history of abortion and repeated breeding.

The clinical, pathological, and immunohistochemical fi ndings associated with a vaginal leiomyosarcoma in a 12-year-

Correspondência: João Paulo Elsen Saut: jpsaut@famev.ufu.br RESUMO: A endometrite subclínica tem efeito negativo no desempenho reprodutivo pois reduz a taxa de prenhez e aumenta o intervalo parto-concepção. Diante disto, objetivou-se avaliar a ocorrência de endometrite subclínica (ES) aos 42 dias pós-parto (dpp) em vacas que foram diagnosticadas com endometrite clínica entre 21 e 28 dpp e tratadas com infusão uterina de oxitetraciclina. Foram utilizadas 54 vacas mestiças leiteiras, divididas em dois grupos: o controle -GC (n=34), composto por vacas com parto e puerpério fisiológico de acordo com o exame clínico proposto e sem nenhum tratamento; e o grupo tratado -GT (n=20), vacas com endometrite clínica tratadas entre 21 e 28 dpp, com infusão uterina (4 g de oxitetraciclina) entre 21 e 28 dpp. Acompanhou-se no dia do parto, 7, 14, 21, 28 e 42 dpp. Realizou-se avaliação dos parâmetros vitais, escore de condição corporal, exame ginecológico e citologia endometrial através da técnica de cytobrush. Aos 42 dpp não houve diferença na porcentagem de muco vaginal limpo (P=0,69) e os dois grupos apresentaram a mesma evolução clínica. Aos 42 dpp houve diferença entre a ocorrência de ES (P<0.001) entre GC (14,8%) e o GT (80,0%), além de apresentar diferença na proporção de neutrófilos (3,3 ± 6,6; 54,6 ± 39,9 -p<0,001), células endometriais (95,9 ± 8,0; 39,6 ± 40 -p0,001), macrófagos (0,1 ± 0,3; 1,1 ± 1,0 -p=0,016), linfócitos (0,6 ± 1,9; 3,9 ± 4,2 -p<0,001) e eosinófilos (0,1 ± 0,2; 0,8 ± 2,0 -p=0,113), respectivamente. Concluiu-se que, apesar das vacas com endometrite clínica apresentarem resposta ao tratamento com funções vitais, involução uterina e secreção vaginal semelhante às vacas com puerpério fisiológico, ainda persistem com uma alta incidência de endometrite subclínica (80%) que representa um efeito negativo no desempenho reprodutivo. Palavras-chave: bovinos; citologia endometrial; cytobrush; inflamação; útero SUBCLINICAL ENDOMETRITIS AFTER TREATMENT IN COWS WITH CLINICAL ENDOMETRITISABSTRACT: Subclinical endometritis causes negative effects on reproductive performance how decreased conception rates and prolonged days open. The objective of this study was to evaluate the occurrence of subclinical endometritis (SE) at 42 days in milk (DIM) in cows that were diagnosed with clinical endometritis between 21 and 28 DIM and treated with uterine infusion of oxytetracycline. 54 crossbreed dairy cows were divided into two groups: GC (n=34), animals that demonstrated physiological parturition and puerperium, based on the proposed clinical evaluation and without being treated; GT (n=20), cows with endometritis received uterine infusion (4 g of oxytetracycline) between 21 and 28 DIM. Cows were examined at parturition and at days 7, 14, 21, 28, and 42 DIM. The following procedures were performed: vital parameters and body condition score evaluation; gynecological exams and endometrial cytology using the cytobrush technique. There was no difference (P=0.69) in the percentage of clear vaginal mucus between groups at 42 DIM, th...