The floristic region of Adjara represents “Hotpoint” of Caucasian which is distinguished with the uniqueness of its it relict Colchis flora.It represents one of the most powerful refuge in western Eurasia , which is not touched by the chilling because has the special geographical location. There are 176 endemic plants spread in southern Colchis, of which 45 ones can be used for some medical treatments. The bioecology and detailed phytochemical content of some medicinal plant populations have not been studied so far. The research objective is to study the phytochemical content of endemic species of Helleborus caucasicus and Helleborus abchasicus spread in southern Colchis. The research method for the phytochemical content included the separation analysis, which was performed by using UPLC-MS (Waters Acquity QDa detector). Three Steroidal glycosides were isolated from the MeOH extract of the plants of Helleborus caucasicus and Helleborus abchasicus: Hellebrigenin-D-glucose, 20 – Hydroxyecdysone and Hydroxyecdysone – 3 glucoside. Three Steroidal glycosides and Hydroxyecdysone -3 glucoside have been isolated from the MeOH extract of Helleborus caucasicus.

There are 176 endemic plants spread in southern Colchis, of which 45  can be used for some medical treatments. The bioecology and detailed phytochemical content of some medicinal plant populations have not  been studied so far. The research objectiveis to study the phytochemical content of endemic species of Helleborus caucasicus, Helleborus abchasicus and Ficaria popovii spread in southern Colchis. The research method for the phytochemical content included separation analysis, whichwas performed byusing UPLC-MS (Waters Acquity QDa detector). Three Steroidal glycosideswere isolated from the MeOH extract of the plants of Helleborus caucasicus and Helleborus abchasicus: Hellebrigenin-D-glucose, 20 – Hydroxyecdysone and Hydroxyecdysone – 3 glucoside. Two saponins (Hederagenin 3-O -α-L-arabino pyranoside, Hederagenin28-O-[α-L-rhamno-pyranosyl(1→4)-β-D-glucopyranosyl-(1→6)]β-D-lucopyranoside) and four flavonoids (kaempferol 3-O-β-ᴅ- (6ʺ-α-ʟ-rhamnopyranosyl)-glucopyranoside (nicotiflorin), apigenin 8-C-β-ᴅ-glucopyranoside (vitexin), luteolin 8-C-β-ᴅ-glucopyranoside (orientin), quercetin 3-O-rutinoside) were  isolated from the tubers and flowers of Ficaria Popovii. Three Steroidal glycosides and Hydroxyecdysone -3 glucoside were isolated from the MeOH extract of Helleborus caucasicus.In addition, two saponins and four flavonoids were isolated from the tubers and leaves of FicariaPopovii.

Adjara (South Kolkheti), located in the southwestern part of Georgia, it is characterized by warm and humid climate and subtropical climate, which is due to the proximity of the Black Sea. Adjara is remarkably rich in the diversity of flora not only in Georgia, but throughout the Caucasus. At present there are 1837 plant species in the flora of Adjara, 72 of which contain essential oils, they are distributed in 22 families and in 52 genera. Plants containing essential oil belongs: Lamiaceae-18 species, Asteraceae-17 types, Apiaceae-9, Cupressaceae-3, Pinaceae-3, Myrtaceae-3. Vital forms are 7 species of trees, 5 species of bushes, 1 species of tree or bushes, 49 species of perennial grass, 6 species of annual grass. According to the geographical coordinates from the sea level up to 0-500 m are spread 36 species containing essential oils, from the sea level up to 500-1000 m 28 species, from the sea level up to 1000 m 2200 m 33 species. According to the origin of species, 48 species are wild relatives, 7 is cultivated as vegetables, 3 species are introduced, 3 species are invasive. According to the percentage content of essential oils there are: 1

Within the framework of this work, the authors conducted studies of plants (tangerine, lemon, orange), for the specifics of their contamination with heavy metals (Pb, Cu, Ba, Sr.). Several groups of correlative dependencies have been identified: between the accumulation of copper in lemon and the defeat of this plant by ticks; lead accumulation through the leaves is especially intense in the immediate vicinity of the road, therefore the amount of lead in the leaves of plants (growing near the road) is much higher; of all citrus oranges are the most suitable for use as a bioindicator of environmental pollution by lead. Keywords: ENVIRONMENTAL POLLUTION, HEAVY METALS, CITRUS FRUITS, TANGERINES, ORANGES, LEMONS, ACCUMULATION, SOILS, LEAVES, ROOT SYSTEM, BIOINDICATOR