Background: Chronic suppurative otitis media (CSOM) is a notorious infection and a major health problem in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causative micro-organisms to ensures prompt clinical recovery and possible complications can thus be avoided. Objective: The of this study was to isolate the organisms associated with CSOM. Materials and Methods: The prospective study was carried out at a tertiary care hospital in a time period of 1 yr (data collection) clinically diagnosed CSOM cases were included in the study.Pus samples from both outpatient and inpatient departments were included.Detailed history was obtained from each patient according to the case proforma. Result: Out of 122 pus samples significant growth was seen in 120(98.36%) samples, were 2(1.63%) sample showed insignificant or no growth. It is mostly seen that aerobic gram negative rods outnumber gram positive cocci organisms in CSOM as reported by some authors 175 which is comparable to the findings in the present study where gram negative rods accounted for 94(78.33%) and gram positive cocci 26(21.66%). Conclusion: Klebsiella pneumoniae (24.16%) was the commonest isolate followed by Pseudomonas aeruginosa (18.33%).