Psidium Guajava, family Myrtaceae and Glycyrrhiza glabra family Leguminosae has been reported to possess antioxidant, antimicrobial, antiinflammatory, antibacterial and antifungal properties. Gauva and Liquiorice extracts have been used to treat antimicrobial infections. It contain several chemicals compound such as flavonoids, tannins, phenols, triterpenes, saponins, to take the benefit of the nature of extract. Psidium Guajava and Glycyrrhiza glabra prepare shampoo incorporating in the herbal constituent base and evaluate this natural herbal shampoo. The herbal shampoo formulations comprising of hydroalcoholic extract of Psidium Guajava and Glycyrrhiza glabra (ethanol:water 80:20v/v), coconut oil, castor oil, cymbopogon citrates oil were prepared and evaluated for physicochemical parameters such as visual appearance, pH, viscosity, % of solids content, foaming capacity and the results showed that the formulation F4 of anti-dandruff herbal shampoo contains all good characters of an ideal shampoo and it was found to be harmless, more effective, ease of manufacturing and economical compared to synthetic antidandruff shampoo.