The synthetic techniques, for the synthesis of Metal oxide nanoparticles involve toxic solvents, high pressure, high-energy and high temperature which may be efficient but not eco-friendly. The synthetic techniques for metal oxide nanoparticles using plants, has several advantages over the chemical synthesis such as cost effectiveness, simplicity as well as compatibility for medical applications. In this part of the study we are using the cynodon dactylon leaf extracts to synthesize titanium dioxide nanoparticles. The properties of TiO 2 include high refractive index, light absorption, non toxicity, chemical stability and relatively low cost production. The cynodon dactylon leaf has reduced the size of TiO 2 nanoparticles. In the present study antibacterial effect of TiO 2 nanoparticles on E. coli strain was analyzed. The well diffusion assay is used to confirm the inhibition zone of bacteria. Further the characteristics of the obtained TiO 2 nanoparticles were studied using XRD, FTIR, Laser Raman spectroscopy, Scanning Electron Microscopic and the results are presented in detail.