The aim of this experiment was to detect the teratogenic effects of difloxacin in Wister Albino rats. Thirty pregnant rats were divided into 3 groups; group (1) behaved as control and received normal saline. Group (2) was administered difloxacin at a dose of 20 mg/kg b. wt and group (3) was administered difloxacin at a dose of 40 mg/kg b. wt orally once daily from 6 th to 15 th day of pregnancy. The study revealed no teratogenic effect at a dose of 20 mg/kg b. wt, while at a dose of 40 mg/kg b.wt has significant teratogenic effect showed by decrease in size, weight, length, retarded growth in fetuses, fetal resorption of treated groups, the incidences of skeletal & visceral abnormalities were increased in treated group (40 mg/kg b. wt). It could be concluded that. Administration of difloxacin during early stage of pregnancy and at high dose induce some fetal abnormalities.